Western blot abreview for Anti-GAS 6 antibody

Average
Abreviews
Application
Western blot
Loading amount
50 cells
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (primary human macrophages)
Specification
primary human macrophages
Treatment
MDP 100ug/ml
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: invitrogen A21109
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000

Detection

Detection method
ODYSSEY
Bands
Specific: 150 kDa
Exposure
1 minute(s) and 0 second(s)
Positive control
Hela

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Submitted Jun 18 2013

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