Anti-GAS 6 antibody (ab227174)
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- References
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Overview
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Product nameAnti-GAS 6 antibody
See all GAS 6 primary antibodies -
DescriptionRabbit polyclonal to GAS 6
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment within Human GAS 6 (internal sequence). The exact sequence is proprietary.
Database link: Q14393 -
Positive control
- WB: HEK-293T and A431 whole cell lysates. ICC/IF: MCF7 cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.75% Glycine, 1.21% Tris -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab227174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 80 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionLigand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.
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Tissue specificityPlasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen.
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Sequence similaritiesContains 4 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 laminin G-like domains. -
Post-translational
modificationsIsoform 1 is proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL.
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 2621 Human
- Omim: 600441 Human
- SwissProt: Q14393 Human
- Unigene: 646346 Human
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Alternative names
- AXL receptor tyrosine kinase ligand antibody
- AXL stimulatory factor antibody
- AXLLG antibody
see all
Images
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Western blot - Anti-GAS 6 antibody (ab227174)All lanes : Anti-GAS 6 antibody (ab227174) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 80 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-GAS 6 antibody (ab227174)Methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for GAS 6 (green) using ab227174 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342.
References
ab227174 has not yet been referenced specifically in any publications.
