Anti-GAS 6 antibody (ab227174)

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Western blot - Anti-GAS 6 antibody (ab227174)
  • Immunocytochemistry/ Immunofluorescence - Anti-GAS 6 antibody (ab227174)

Key features and details

  • Rabbit polyclonal to GAS 6
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-GAS 6 antibody
    See all GAS 6 primary antibodies

  • Description

    Rabbit polyclonal to GAS 6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human GAS 6 (internal sequence). The exact sequence is proprietary.
    Database link: Q14393

  • Positive control

    • WB: HEK-293T and A431 whole cell lysates. ICC/IF: MCF7 cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.

  • Tissue specificity

    Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen.

  • Sequence similarities

    Contains 4 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 2 laminin G-like domains.

  • Post-translational
    modifications

    Isoform 1 is proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL.
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • AXL receptor tyrosine kinase ligand antibody
      • AXL stimulatory factor antibody
      • AXLLG antibody
      • AXS F antibody
      • AXSF antibody
      • DKFZp666G247 antibody
      • FLJ34709 antibody
      • Gas 6 antibody
      • GAS-6 antibody
      • Gas6 antibody
      • GAS6_HUMAN antibody
      • Growth arrest specific 6 antibody
      • Growth arrest-specific protein 6 antibody
      • Growth-arrest-specific protein 6 antibody
      • Growth-arrest-specific protein 6 precursor antibody
      see all

    Images

    • Western blot - Anti-GAS 6 antibody (ab227174)
      Western blot - Anti-GAS 6 antibody (ab227174)
      All lanes : Anti-GAS 6 antibody (ab227174) at 1/1000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG

      Predicted band size: 80 kDa



      7.5% SDS-PAGE gel.

    • Immunocytochemistry/ Immunofluorescence - Anti-GAS 6 antibody (ab227174)
      Immunocytochemistry/ Immunofluorescence - Anti-GAS 6 antibody (ab227174)

      Methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for GAS 6 (green) using ab227174 at 1/500 dilution in ICC/IF.

      Nuclear counterstain: Hoechst 33342.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab227174? Please let us know so that we can cite the reference in this datasheet.

    ab227174 has not yet been referenced specifically in any publications.

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