Anti-GAS 6 antibody (ab67099)
Key features and details
- Mouse polyclonal to GAS 6
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GAS 6 antibody
See all GAS 6 primary antibodies -
Description
Mouse polyclonal to GAS 6 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human GAS 6 aa 1-700. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa). |
Target
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Function
Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. -
Tissue specificity
Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen. -
Sequence similarities
Contains 4 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 laminin G-like domains. -
Post-translational
modificationsIsoform 1 is proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL.
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2621 Human
- Omim: 600441 Human
- SwissProt: Q14393 Human
- Unigene: 646346 Human
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Alternative names
- AXL receptor tyrosine kinase ligand antibody
- AXL stimulatory factor antibody
- AXLLG antibody
see all
Images
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All lanes : Anti-GAS 6 antibody (ab67099) at 1/500 dilution
Lane 1 : GAS 6 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg/ml per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 80 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab67099 has not yet been referenced specifically in any publications.