Anti-GAS 6 antibody (ab67099)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GAS 6 antibody
See all GAS 6 primary antibodies -
Description
Mouse polyclonal to GAS 6 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein, corresponding to amino acids 1-678 of Human GAS 6
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Positive control
- 293T cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab67099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Application notes
WB: 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).
Detection Antibody Pair.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Detection Antibody Pair.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. -
Tissue specificity
Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen. -
Sequence similarities
Contains 4 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 laminin G-like domains. -
Post-translational
modificationsIsoform 1 is proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL.
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2621 Human
- Omim: 600441 Human
- SwissProt: Q14393 Human
- Unigene: 646346 Human
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Alternative names
- AXL receptor tyrosine kinase ligand antibody
- AXL stimulatory factor antibody
- AXLLG antibody
see all
Images
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Western blot - Anti-GAS 6 antibody (ab67099)All lanes : Anti-GAS 6 antibody (ab67099) at 1/500 dilution
Lane 1 : GAS 6 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg/ml per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 80 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
ab67099 has not yet been referenced specifically in any publications.
