Overview

  • Product name
    Anti-GAS-7 antibody [EPR11129(B)]
    See all GAS-7 primary antibodies
  • Description
    Rabbit monoclonal [EPR11129(B)] to GAS-7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human GAS-7 aa 450-550.
    Database link: O60861

  • Positive control
    • Human fetal brain, Human cerebellum, Mouse and Rat brain lysates; Human brain tissue; U87-MG cells.
  • General notes

     

     

    Previously labelled as Growth Arrest Specific Protein 7. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab168370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 39-54 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      May play a role in promoting maturation and morphological differentiation of cerebellar neurons.
    • Involvement in disease
      Note=A chromosomal aberration involving GAS7 is found in acute myeloid leukemia. Translocation t(11;17)(q23;p13) with MLL/HRX.
    • Sequence similarities
      Contains 1 FCH domain.
      Contains 1 SH3 domain.
      Contains 1 WW domain.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • GAS 7 antibody
      • GAS-7 antibody
      • Gas7 antibody
      • GAS7_HUMAN antibody
      • Growth arrest specific 7 antibody
      • Growth Arrest Specific Protein 7 antibody
      • Growth arrest-specific protein 7 antibody
      • KIAA0394 antibody
      • MGC1348 antibody
      • MLL/GAS7 antibody
      • MLL/GAS7 fusion protein antibody
      see all

    Images

    • Immunofluorescent analysis of U87-MG cells cells labeling GAS-7 with ab168370 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling GAS-7 with ab168370 at 1/100 dilution.

    • All lanes : Anti-GAS-7 antibody [EPR11129(B)] (ab168370) at 1/1000 dilution

      Lane 1 : mouse brain lysate
      Lane 2 : rat brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 54 kDa

    • All lanes : Anti-GAS-7 antibody [EPR11129(B)] (ab168370) at 1/1000 dilution

      Lane 1 : fetal brain lysate
      Lane 2 : Human cerebellum lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 54 kDa

    References

    ab168370 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (human melanoma cell)
    Total protein in input
    500 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    human melanoma cell

    Jintana Saowapa

    Verified customer

    Submitted May 28 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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