Recombinant Anti-GAS-7 antibody [EPR11129(B)] (ab168370)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11129(B)] to GAS-7
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GAS-7 antibody [EPR11129(B)]
See all GAS-7 primary antibodies -
Description
Rabbit monoclonal [EPR11129(B)] to GAS-7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human GAS-7 aa 450-550.
Database link: O60861 -
Positive control
- Human fetal brain, Human cerebellum, Mouse and Rat brain lysates; Human brain tissue; U87-MG cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11129(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 39-54 kDa (predicted molecular weight: 54 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/50 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 39-54 kDa (predicted molecular weight: 54 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/100. |
Target
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Function
May play a role in promoting maturation and morphological differentiation of cerebellar neurons. -
Involvement in disease
Note=A chromosomal aberration involving GAS7 is found in acute myeloid leukemia. Translocation t(11;17)(q23;p13) with MLL/HRX. -
Sequence similarities
Contains 1 FCH domain.
Contains 1 SH3 domain.
Contains 1 WW domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8522 Human
- Omim: 603127 Human
- SwissProt: O60861 Human
- SwissProt: Q60780 Mouse
- SwissProt: O55148 Rat
- Unigene: 258855 Human
- Unigene: 462214 Human
- Unigene: 152121 Mouse
see all -
Alternative names
- GAS 7 antibody
- GAS-7 antibody
- Gas7 antibody
see all
Images
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All lanes : Anti-GAS-7 antibody [EPR11129(B)] (ab168370) at 1/1000 dilution
Lane 1 : mouse brain tissue lysate
Lane 2 : rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 54 kDa -
Immunofluorescent analysis of U87-MG cells cells labeling GAS-7 with ab168370 at 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling GAS-7 with ab168370 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-GAS-7 antibody [EPR11129(B)] (ab168370) at 1/1000 dilution
Lane 1 : fetal brain lysate
Lane 2 : Human cerebellum lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 54 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab168370 has not yet been referenced specifically in any publications.