Key features and details
- Rabbit polyclonal to Gas1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Gas1 antibody
See all Gas1 primary antibodies
DescriptionRabbit polyclonal to Gas1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- WB: SH-SY5Y and HEK-293 whole cell lysate. ICC/IF: HeLa cells. IHC-P: Human pancreas and skin tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionSpecific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.
Cellular localizationCell membrane.
- Information by UniProt
- GAS-1 antibody
- Gas1 antibody
- GAS1_HUMAN antibody
All lanes : Anti-Gas1 antibody (ab236618) at 1/500 dilution
Lane 1 : SH-SH5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Paraffin-embedded human skin tissue stained for Gas1 with ab236618 at a 1/100 dilution in immunohistochemical analysis.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Gas1 (Green) using ab236618 at a 1/133 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
Paraffin-embedded human pancreas tissue stained for Gas1 with ab236618 at a 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236618 has not yet been referenced specifically in any publications.