Overview

  • Product name

    Anti-Gas1 antibody
  • Description

    Rabbit polyclonal to Gas1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Gas1 aa 248-318.
    Sequence:

    LGNGPGSSGSDGGLDDYYDEDYDDEQRTGGAGGEQPLDDDDGVPHPPRPG SGAAASGGRGDLPYGPGRRSS


    Database link: P54826

  • Positive control

    • WB: SH-SY5Y and HEK-293 whole cell lysate. ICC/IF: HeLa cells. IHC-P: Human pancreas and skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab236618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000.
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GAS-1 antibody
    • Gas1 antibody
    • GAS1_HUMAN antibody
    • Growth arrest-specific protein 1 antibody
    • Growth-arrest-specific protein 1 precursor antibody
    see all

Images

  • All lanes : Anti-Gas1 antibody (ab236618) at 1/500 dilution

    Lane 1 : SH-SH5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
  • Paraffin-embedded human skin tissue stained for Gas1 with ab236618 at a 1/100 dilution in immunohistochemical analysis.

  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Gas1 (Green) using ab236618 at a 1/133 dilution in ICC/IF.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

  • Paraffin-embedded human pancreas tissue stained for Gas1 with ab236618 at a 1/100 dilution in immunohistochemical analysis.

References

ab236618 has not yet been referenced specifically in any publications.

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