Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1742(2)] to GAS2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
Product nameAnti-GAS2 antibody [EPR1742(2)]
See all GAS2 primary antibodies
DescriptionRabbit monoclonal [EPR1742(2)] to GAS2
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human GAS2 aa 1-100 (N terminal). The exact sequence is proprietary.
- HepG2, fetal liver, fetal kidney, and Jurkat lysates, Human stomach tissue
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process.
Tissue specificityUbiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen.
Sequence similaritiesBelongs to the GAS2 family.
Contains 1 CH (calponin-homology) domain.
Contains 1 GAR domain.
Developmental stageSpecifically expressed at growth arrest.
modificationsCleaved, during apoptosis, on a specific aspartic residue by caspases.
Phosphorylated on serine residues during the G0-G1 transition phase.
Cellular localizationCytoplasm > cytoskeleton. Membrane. Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles.
- Information by UniProt
- GAS 2 antibody
- GAS-2 antibody
- Gas2 antibody
All lanes : Anti-GAS2 antibody [EPR1742(2)] (ab109762) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Immunohistochemical analysis of paraffin-embedded Human stomach tissue using ab109762 at a dilution of 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab109762 has been referenced in 2 publications.
- Shukla S et al. Aberrant Activation of a Gastrointestinal Transcriptional Circuit in Prostate Cancer Mediates Castration Resistance. Cancer Cell 32:792-806.e7 (2017). PubMed: 29153843
- Sun L et al. GAS2-Calpain2 axis contributes to the growth of leukemic cells. Acta Biochim Biophys Sin (Shanghai) 47:795-804 (2015). PubMed: 26358320