Anti-GAS41 antibody (ab205018)
Key features and details
- Rabbit polyclonal to GAS41
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-GAS41 antibody
See all GAS41 primary antibodies -
Description
Rabbit polyclonal to GAS41 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
-
Immunogen
Synthetic peptide within Human GAS41 aa 150 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_006521.1).
Database link: O95619 -
Positive control
- Hela, Jurkat and 293T whole cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
|
|
| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 26 kDa.
|
| Notes |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
|
WB
1/2000 - 1/10000. Predicted molecular weight: 26 kDa. |
Target
-
Function
Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. -
Tissue specificity
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. -
Sequence similarities
Contains 1 YEATS domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 8089 Human
- Omim: 602116 Human
- SwissProt: O95619 Human
- Unigene: 4029 Human
-
Alternative names
- 4930573H17Rik antibody
- B230215M10Rik antibody
- GAS 41 antibody
see all
Images
-
Western blot - Anti-GAS41 antibody (ab205018)All lanes : Anti-GAS41 antibody (ab205018) at 0.1 µg/ml
Lane 1 : Hela whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-GAS41 antibody (ab205018)Detection of GAS41 in Immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab205018 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
-
Datasheet download
References (1)
ab205018 has been referenced in 1 publication.
- Ji S et al. YEATS Domain Containing 4 Promotes Gastric Cancer Cell Proliferation and Mediates Tumor Progression via Activating the Wnt/ß-Catenin Signaling Pathway. Oncol Res 25:1633-1641 (2017). PubMed: 28251887
