Overview

  • Product name

    Anti-Gastric Mucin/MUC-6 antibody [EPR20623]
    See all Gastric Mucin/MUC-6 primary antibodies
  • Description

    Rabbit monoclonal [EPR20623] to Gastric Mucin/MUC-6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Gastric Mucin/MUC-6 aa 1950-2050. The exact sequence is proprietary.
    Database link: Q6W4X9

  • Positive control

    • IHC-P: Human stomach and duodenum tissue. Human gastric cancer tissue.
  • General notes

    Previously labelled as Gastric Mucin.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab223846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    May provide a mechanism for modulation of the composition of the protective mucus layer related to acid secretion or the presence of bacteria and noxious agents in the lumen. Play an important role in the cytoprotection of epithelial surfaces and are used as tumor markers in a variety of cancers. May play a role in epithelial organogenesis.
  • Tissue specificity

    Expressed in the regenerative zone of gastric antrum, gastric body mucosa and gastric incisura mucosa. Expressed in the deeper mucous glands of gastric antrum. Overexpressed in Helicobacter pylori infected gastric epithelium. Highly expressed in duodenal Brunner's glands, gall bladder, seminal vesicle, pancreatic centroacinar cells and ducts, and periductal glands of the common bile duct.
  • Sequence similarities

    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 TIL (trypsin inhibitory-like) domain.
    Contains 3 VWFD domains.
  • Developmental stage

    Early expressed in fetal development and was observed in Brunner's glands and pancreatic ducts at 18-19 weeks and in gastric glands at 20 weeks of gestation. Expressed transiently in the nephrogenic zone of the kidney in the early mid-trimester of development. Detected in the epithelium of ureteric buds at 13 weeks and at lower levels from 17 to 23 weeks of gestation.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Gastric mucin 6 antibody
    • Gastric Mucin antibody
    • Gastric mucin-6 antibody
    • MUC 6 antibody
    • MUC-6 antibody
    • Muc6 antibody
    • MUC6 Fragment antibody
    • MUC6 mucin antibody
    • MUC6 mucin Fragment antibody
    • MUC6_HUMAN antibody
    • Mucin 6 antibody
    • Mucin 6 gastric antibody
    • Mucin 6 oligomeric mucus/gel forming antibody
    • Mucin glycoprotein Fragment antibody
    • Mucin-6 antibody
    • Secretory mucin MUC6 Fragment antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Gastric Mucin/MUC-6 with ab223846 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human stomach (PMID:17227128). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded human duodenum tissue labeling Gastric Mucin/MUC-6 with ab223846 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on Brunner's glands of human duodenum (PMID:10681391). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling Gastric Mucin/MUC-6 with ab223846 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human gastric cancer (PMID: 14557879). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

References

ab223846 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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