Anti-Gastrin antibody (ab53085)
Key features and details
- Rabbit polyclonal to Gastrin
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Gastrin antibody
See all Gastrin primary antibodies -
Description
Rabbit polyclonal to Gastrin -
Host species
Rabbit -
Specificity
ab53085 detects endogenous levels of total Gastrin protein. The immunogen for this antibody has high alignment to CKK. It is therefore predicted to detect CKK as well as Gastrin, though this has not been tested experimentally. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide derived from Human Gastrin
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
Target
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Function
Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. -
Sequence similarities
Belongs to the gastrin/cholecystokinin family. -
Post-translational
modificationsTwo different processing pathways probably exist in antral G-cells. In the dominant pathway progastrin is cleaved at three sites resulting in two major bioactive gastrins, gastrin-34 and gastrin-17. In the putative alternative pathway, progastrin may be processed only at the most C-terminal dibasic site resulting in the synthesis of gastrin-71.
Sulfation enhances proteolytic processing, and blocks peptide degradation. Levels of sulfation differ between proteolytically-cleaved gastrins. Thus, gastrin-6 is almost 73% sulfated, whereas the larger gastrins are less than 50% sulfated. Sulfation levels are also tissue-specific. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 14459 Mouse
- SwissProt: P48757 Mouse
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Alternative names
- Component II antibody
- G14 antibody
- G17 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab53085 has been referenced in 3 publications.
- Zhao Y et al. Gastrin/CCK-B Receptor Signaling Promotes Cell Invasion and Metastasis by Upregulating MMP-2 and VEGF Expression in Gastric Cancer. J Cancer 13:134-145 (2022). PubMed: 34976177
- Miao ZF et al. A Metformin-Responsive Metabolic Pathway Controls Distinct Steps in Gastric Progenitor Fate Decisions and Maturation. Cell Stem Cell 26:910-925.e6 (2020). PubMed: 32243780
- Lavoie EG et al. Ectonucleotidases in the digestive system: focus on NTPDase3 localization. Am J Physiol Gastrointest Liver Physiol 300:G608-20 (2011). IHC-Fr ; Mouse . PubMed: 21233276