• Product name

  • Description

    Rabbit polyclonal to Gastrin
  • Host species

  • Specificity

    ab53085 detects endogenous levels of total Gastrin protein. The immunogen for this antibody has high alignment to CKK. It is therefore predicted to detect CKK as well as Gastrin, though this has not been tested experimentally.
  • Tested applications

    Suitable for: IHC-Fr, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from Human Gastrin

  • Positive control

    • NIH/3T3 cell extract



Our Abpromise guarantee covers the use of ab53085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 21233276
WB 1/500 - 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
ELISA 1/10000.


  • Function

    Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
  • Sequence similarities

    Belongs to the gastrin/cholecystokinin family.
  • Post-translational

    Two different processing pathways probably exist in antral G-cells. In the dominant pathway progastrin is cleaved at three sites resulting in two major bioactive gastrins, gastrin-34 and gastrin-17. In the putative alternative pathway, progastrin may be processed only at the most C-terminal dibasic site resulting in the synthesis of gastrin-71.
    Sulfation enhances proteolytic processing, and blocks peptide degradation. Levels of sulfation differ between proteolytically-cleaved gastrins. Thus, gastrin-6 is almost 73% sulfated, whereas the larger gastrins are less than 50% sulfated. Sulfation levels are also tissue-specific.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Component II antibody
    • G14 antibody
    • G17 antibody
    • G34 antibody
    • G52 antibody
    • G6 antibody
    • GAS antibody
    • Gast antibody
    • GAST_HUMAN antibody
    • Gastrin antibody
    • Gastrin component I antibody
    • Gastrin component II antibody
    • Gastrin component III antibody
    • Gastrin precursor antibody
    • Gastrin-14 antibody
    • Gastrin-17 antibody
    • Gastrin-34 antibody
    • Gastrin-52 antibody
    • Gastrin-6 antibody
    • Gastrin-71 antibody
    see all


  • All lanes : Anti-Gastrin antibody (ab53085) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extract
    Lane 2 : NIH/3T3 cell extract with peptide

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


This product has been referenced in:

  • Lavoie EG  et al. Ectonucleotidases in the digestive system: focus on NTPDase3 localization. Am J Physiol Gastrointest Liver Physiol 300:G608-20 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21233276) »
See 1 Publication for this product

Customer reviews and Q&As


Thank you for your enquiry.

I am sorry to confirm that the crossreactivity of this ab53085 Gastrin antibody with CKK has not been specifically tested in the laboratory.

However, alignment of the immunogen sequence of the antibody with mouse CKK (http://www.uniprot.org/uniprot/P09240) shows a 100% identity in a 10-residue overlap with a score of 52.0 and gap frequency of 0.0%. Therefore, this antibody is predicted to crossreact with mouse CKK.

I am sorry this is not better news for you on this occasion. If you have any further questions, please do not hesitate to contact us.

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