Key features and details
- Rabbit monoclonal [SP318] to Gastrin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Gastrin antibody [SP318]
See all Gastrin primary antibodies
DescriptionRabbit monoclonal [SP318] to Gastrin
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Gastrin (internal sequence). The exact sequence is proprietary.
Database link: P01350
- IHC-P: Human stomach tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Our Abpromise guarantee covers the use of ab223501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionGastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
Sequence similaritiesBelongs to the gastrin/cholecystokinin family.
modificationsTwo different processing pathways probably exist in antral G-cells. In the dominant pathway progastrin is cleaved at three sites resulting in two major bioactive gastrins, gastrin-34 and gastrin-17. In the putative alternative pathway, progastrin may be processed only at the most C-terminal dibasic site resulting in the synthesis of gastrin-71.
Sulfation enhances proteolytic processing, and blocks peptide degradation. Levels of sulfation differ between proteolytically-cleaved gastrins. Thus, gastrin-6 is almost 73% sulfated, whereas the larger gastrins are less than 50% sulfated. Sulfation levels are also tissue-specific.
- Information by UniProt
- Component II antibody
- G14 antibody
- G17 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling Gastrin with ab223501 at 1/100 dilution (1.08 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadic positive staining on the human stomach, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab223501 for 10 mins at room temperature.
Paraffin-embedded human stomach tissue stained for Gastrin using ab223501 at 1/100 dilution in immunohistochemical analysis.
ab223501 has not yet been referenced specifically in any publications.