Overview

  • Product name
    Anti-Gastrin Releasing Peptide antibody
    See all Gastrin Releasing Peptide primary antibodies
  • Description
    Rabbit polyclonal to Gastrin Releasing Peptide
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human) conjugated to BSA.

Properties

Applications

Our Abpromise guarantee covers the use of ab14390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.

Target

  • Function
    GRP stimulates gastrin release as well as other gastrointestinal hormones. Operates as a negative feedback regulating fear and established a causal relationship between GRP-receptor gene expression, long-term potentiation, and amygdala-dependent memory for fear.
  • Sequence similarities
    Belongs to the bombesin/neuromedin-B/ranatensin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BN antibody
    • Bombesin antibody
    • GRP antibody
    • GRP-10 antibody
    • GRP_HUMAN antibody
    • GRP10 antibody
    • Neuromedin-C antibody
    • NeuromedinC antibody
    • Pre progastrin releasing peptide antibody
    • PreproGRP antibody
    • ProGRP antibody
    see all

References

ab14390 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for your enquiry. Since this antibody is a polyclonal raised to full-length GRP, it will be a mix of IgGs that recognize different epitopes along the entire length of the protein. Therefore, it should recognize alternatively spliced versions of the protein, although this hasn't been specifically tested. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. Unfortunately, we do not have a specific ELISA protocol for this antibody other than the 1:1000 dilution. I would suggest referring to our general ELISA protocol which I have included below (this protocol is also accessible by clicking on the protocols tab located on the online datasheet). If you have any additional questions, please contact us again. 1. Dilute the antigen to a final concentration of 20 µg/ml in PBS. Coat the wells of a PVC microtiter plate with the antigen by pipeting 50 µl of the antigen dilution per well. 2. Cover the plate with an adhesive plastic and incubate for 2 h at room temperature. 3. Remove the coating solution and wash the plate twice by filling the wells with 300 µl PBS. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel. 4. Block the remaining protein-binding sites in the coated wells by adding 300 µl blocking buffer, 5% non fat dry milk/PBS, per well. 5. Cover the plate with an adhesive plastic and incubate for at least 2 h at room temperature or, if more convenient, overnight at 4°C. 6. Wash the plate twice with PBS. 7. Make 10-fold dilutions (1:100, 1:1,000, 1:10,000, 1:100,000 and 1:1,000,000) of serum and pre-immune serum (the latter as a negative control) in blocking buffer. Add 50 µl of each dilution to an antigen-coated well. 8. Cover the plate with an adhesive plastic and incubate for 2 h at room temperature. 9. Wash the plate four times with PBS. 10. Add 50 µl of secondary antispecies antibody conjugated to alkaline phosphatase, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. 11. Cover the plate with an adhesive plastic and incubate for 2 h at room temperature. 12. Wash the plate four times with PBS. 13. Dissolve p-Nitrophenyl phosphate at a concentration of 1 mg/ml in substrate buffer (1M diethanolamine, 0.5 mM MgCl2, pH 9.8). Add 50 µl of the substrate solution per well with a multichannel pipet or a multipipet. 14. Measure the absorbance at 405 nm, using a microtiter plate spectrophotometer. Perform an end-point measurement after 1 h. 15. Calculate the titer of the sera. The titer can be defined as the dilution of serum giving an optical density (OD) of 0.2 above the background of the ELISA after a 1-h reaction.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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