• Product name

    Anti-Gastrin Releasing Peptide antibody
    See all Gastrin Releasing Peptide primary antibodies
  • Description

    Rabbit polyclonal to Gastrin Releasing Peptide
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-FoFr, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic GRP conjugated to BSA by a Glutaraldehyde linker.

  • Positive control

    • Pulmonary neuroendocrine cells



Our Abpromise guarantee covers the use of ab22623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/20.
IHC-FoFr 1/1000.
IHC-P Use at an assay dependent concentration. PubMed: 22411014
IHC-Fr 1/5000.



  • ab22623 at a 1/1000 dilution staining mouse brain (SCN region) by Immunohistochemistry (Formalin fixed sections). The antibody was incubated with the tissue for 16 hours and then detected using an Alexa-Fluor® 488 conjuaged donkey anti-rabbit polyclonal antibody. The mouse SCN region was isolated by stain with Guinea Pig anti-Vasoactive Intestinal Peptide (VIP) antibody.

    This image is courtesy of an Abreview by Gui-lan Yao submitted on 30 November 2005.

    See Abreview


This product has been referenced in:

  • Tattersall M  et al. Expression of gastrin-releasing peptide is increased by prolonged stretch of human myometrium, and antagonists of its receptor inhibit contractility. J Physiol 590:2081-93 (2012). IHC-P ; Human . Read more (PubMed: 22411014) »
  • Rovsing L  et al. A neuroanatomical and physiological study of the non-image forming visual system of the cone-rod homeobox gene (Crx) knock out mouse. Brain Res 1343:54-65 (2010). IHC-Fr ; Mouse . Read more (PubMed: 20438719) »
See all 4 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A



In order for you to successfully use a conjugation kit such as ab102925 with an antibody that is from whole serum (such as the GRP antibody such as ab22623) you will first need to purify the antibody to remove additional proteins that the conjugate might bind to. We do have a purification kit ab102784 that is compabitible with the Easylink kits. This will remove the unwanted substances and allow you to test the concentration of the antibody at the end since it will be purified. Please let me know if I can be of any further assistance.

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I am sorry but for neat serum in particular, there will not be any concentration indicated at all. Even trying to measure it via NanoDrop is not useful simply
because neat serum is typically too “dirty” (extremely protein rich and too many interfering factors) to get an accurate read out. I am sorry I cannot offer you more specific information.

Thank you for your email and have a nice day!

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Thank you for contacting us,

For ab70218:
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For ab22623:
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For ab16007:
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With regards to ab4648, I have contacted the lab to check whether the antibody has been tested in live cells and will get back to you as soon as I get an answer.

Please do not hesitate to contact us if you have any more questions.

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Thank you for contacting Abcam. I have looked at all of the Gastrin Releasing Peptide antibodies in our catalogue and they are all polyclonal raised against the full length (148aa) protein. Therefore I cannot guarantee that any if they will be able to recognize on the 27aa native GRP. We have one Neuromedin C antibody that does recognize the sequence between aa41-50 and that is ab48610. For Retinoic acid, we have several in our catalogue depending if yo wish to detect the alpha, beta or gamma. These include ab28767, ab53161 and 97569 and they all work in at least two of the applications that you specified. I could not find anything in our catalogue for human 9-cis Retinoic acid but if I do find anything, I will pass that information on. If there is anything I can help you with, please let me know.

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Thank you for getting back to me. The following website has a great dot blotting protocol: http://www.westernblotting.org/dot%20blot%20protocol.htm I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Thank you for your enquiry. Gastrin Releasing Peptide antibody (ab22623) has yet to be tested by western blotting. However, according to the SwissProt entry (P63153) the predicted molecular weight of porcine Gastrin Releasing Peptide is 2.8KDa. I would therefore advise you perform a dot blot rather than electrophoresis as a protein of this size will be very difficult to analyse by electrophoresis.

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Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Tissue sections (mouse brain SCN region)
mouse brain SCN region
Antigen retrieval step
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%

Dr. Gui-Lan Yao

Verified customer

Submitted Nov 30 2005


According to UnitProt the MW of GRP is 2806 Da [This is the MW of the unprocessed precursor], Please let us know if you need any further information,

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