Key features and details
- Rabbit polyclonal to Gastrokine 1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Product nameAnti-Gastrokine 1 antibody
See all Gastrokine 1 primary antibodies
DescriptionRabbit polyclonal to Gastrokine 1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human, Pig
Recombinant fragment corresponding to Human Gastrokine 1 aa 22-199. N-terminal His-tag and GST-tag; Expressed in E.coli.
AGLLGVFLAPALANYNINVNDDNNNAGSGQQSVSVNNEHNVANVDNNNGW DSWNSIWDYGNGFAATRLFQKKTCIVHKMNKEVMPSIQSLDALVKEKKLQ GKGPGGPPPKGLMYSVNPNKVDDLSKFGKNIANMCRGIPTYMAEEMQEAS LFFYSGTCYTTSVLWIVDISFCGDTVEN
Database link: Q9NS71
- IHC-P: Human kidney, stomach, pancreas, prostate and liver tissue. WB: Recombinant human Gastrokine 1 protein; Pig and rat stomach tissue lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml.|
FunctionHas mitogenic activity and may be involved in maintaining the integrity of the gastric mucosal epithelium.
Tissue specificityExpressed in stomach. No expression is detected in cancer tissue or gastric cancer cell lines.
Sequence similaritiesBelongs to the gastrokine family.
Contains 1 BRICHOS domain.
- Information by UniProt
- 18 kDa antrum mucosa protein antibody
- AMP 18 antibody
- AMP-18 antibody
All lanes : Anti-Gastrokine 1 antibody (ab231952) at 1 µg/ml
Lane 1 : Pig stomach tissue lysate.
Lane 2 : Rat stomach tissue lysate.
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Developed using the ECL technique.
Anti-Gastrokine 1 antibody (ab231952) at 1 µg/ml + Recombinant human Gastrokine 1 protein.
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Developed using the ECL technique.
Formalin-fixed, paraffin-embedded human liver tissue stained for Gastrokine 1 using ab231952 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human prostate tissue stained for Gastrokine 1 using ab231952 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human pancreas tissue stained for Gastrokine 1 using ab231952 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human stomach tissue stained for Gastrokine 1 using ab231952 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Gastrokine 1 using ab231952 at 20µg/mL in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231952 has not yet been referenced specifically in any publications.