Western blot abreview for Anti-GATA1 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (human iPSC derived hematopoietic progenitor cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Specification
human iPSC derived hematopoietic progenitor cells
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Li Cor blocking buffer in TBS
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: LiCOR 800
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
LiCOR
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 45 kDa

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Submitted Jun 01 2018

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