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    gata1-antibody-ab40847.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-GATA1 antibody (ab40847)

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Western blot - Anti-GATA1 antibody (ab40847)
  • Immunocytochemistry/ Immunofluorescence - Anti-GATA1 antibody (ab40847)
  • Flow Cytometry - Anti-GATA1 antibody (ab40847)
  • Immunocytochemistry/ Immunofluorescence - Anti-GATA1 antibody (ab40847)
  • Western blot - Anti-GATA1 antibody (ab40847)

Key features and details

  • Goat polyclonal to GATA1
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-GATA1 antibody [EPR17362] - ChIP Grade (ab181544)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-GATA1 antibody
    See all GATA1 primary antibodies
  • Description

    Goat polyclonal to GATA1
  • Host species

    Goat
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Synthetic peptide corresponding to Human GATA1 aa 65-76 (internal sequence).
    Sequence:

    DAEAYRHSPVFQ


    Database link: P15976
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules
    • Developmental Biology
    • Organogenesis
    • Hematopoietic system development

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human GATA1 protein (ab204189)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab40847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 10 µg/ml.
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 43 kDa.

1 hour primary incubation is recommended for this product.

Notes
Flow Cyt
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 10 µg/ml.
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 43 kDa.

1 hour primary incubation is recommended for this product.

Target

  • Function

    Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.
  • Tissue specificity

    Erythrocytes.
  • Involvement in disease

    Defects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.
    Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050]; also knwon as thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. XLTT consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced hemoglobin chain synthesis resembling that of beta-thalassemia minor.
    Defects in GATA1 are the cause of anemia without thrombocytopenia X-linked (XLAWT) [MIM:300835]. XLAWT is a form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals.
  • Sequence similarities

    Contains 2 GATA-type zinc fingers.
  • Domain

    The two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding.
  • Post-translational
    modifications

    Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.
    Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation by SUMO1 has no effect on transcriptional activity.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P15976 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2623 Human
    • Entrez Gene: 14460 Mouse
    • Entrez Gene: 25172 Rat
    • Omim: 305371 Human
    • SwissProt: P15976 Human
    • SwissProt: P17679 Mouse
    • SwissProt: P43429 Rat
    • Unigene: 765 Human
    • Unigene: 335973 Mouse
    • Unigene: 10024 Rat
    see all
  • Alternative names

    • Anemia, X-linked, without thrombocytopenia, included antibody
    • ERYF 1 antibody
    • Eryf1 antibody
    • Erythroid transcription factor antibody
    • Erythrold transcription factor 1 antibody
    • GATA 1 antibody
    • GATA binding factor 1 antibody
    • GATA binding protein 1 (globin transcription factor 1) antibody
    • GATA binding protein 1 antibody
    • GATA-1 antibody
    • GATA-binding factor 1 antibody
    • GATA1 antibody
    • GATA1_HUMAN antibody
    • GF 1 antibody
    • GF-1 antibody
    • GF1 antibody
    • Globin transcription factor 1 antibody
    • NF E1 antibody
    • NF E1 DNA binding protein antibody
    • NF-E1 DNA-binding protein antibody
    • NFE 1 antibody
    • NFE1 antibody
    • Nuclear factor erythroid 1 antibody
    • Transcription factor GATA1 antibody
    • XLANP antibody
    • XLTDA antibody
    • XLTT antibody
    see all

Images

  • Western blot - Anti-GATA1 antibody (ab40847)
    Western blot - Anti-GATA1 antibody (ab40847)
    All lanes : Anti-GATA1 antibody (ab40847) at 1 µg/ml

    Lane 1 : K562 nuclear cell lysate
    Lane 2 : Human Hippocampus lysate

    Developed using the ECL technique.

    Predicted band size: 43 kDa



    Negative Control: Human Hippocampus Lysate.

  • Immunocytochemistry/ Immunofluorescence - Anti-GATA1 antibody (ab40847)
    Immunocytochemistry/ Immunofluorescence - Anti-GATA1 antibody (ab40847)

    Immunocytochemsitry/Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% Triton-permeabilized HeLa cells staining GATA1 with ab40847 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2ug/ml (green). DAPI was used as a nuclear counterstain (blue) and actin filaments were stained with phalloidin (red).

    Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

  • Flow Cytometry - Anti-GATA1 antibody (ab40847)
    Flow Cytometry - Anti-GATA1 antibody (ab40847)

    Flow cytometric analysis of paraformaldehyde fixed, 0.15% triton-permeablized K562 cells labelling GATA1 with ab40847 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody (1ug/ml) (blue). Primary incubation carried out for 1hr.

    IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-GATA1 antibody (ab40847)
    Immunocytochemistry/ Immunofluorescence - Anti-GATA1 antibody (ab40847)

    Immunocytochemsitry/Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% Triton-permeabilized NIH3T3 cells staining GATA1 with ab40847 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2ug/ml (green). Primary incubation was carried out for 1 hour. DAPI was used as a nuclear counterstain (blue).

    Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

  • Western blot - Anti-GATA1 antibody (ab40847)
    Western blot - Anti-GATA1 antibody (ab40847)
    Anti-GATA1 antibody (ab40847) at 0.3 µg/ml + Human PBMC lysate (35µg protein in RIPA buffer).

    Predicted band size: 43 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab40847? Please let us know so that we can cite the reference in this datasheet.

ab40847 has not yet been referenced specifically in any publications.

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