Anti-GATA1 antibody (ab40847)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-GATA1 antibody
See all GATA1 primary antibodies -
DescriptionGoat polyclonal to GATA1
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Host speciesGoat
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Tested applicationsSuitable for: ELISA, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
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Immunogen
Synthetic peptide:
DAEAYRHSPVFQ
, corresponding to Internal sequence amino acids 65-76 of Human GATA1
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab40847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | ||
| WB |
Application notes
Peptide ELISA: Antibody detection limit dilution 1:16,000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionTranscriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.
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Tissue specificityErythrocytes.
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Involvement in diseaseDefects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.
Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050]; also knwon as thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. XLTT consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced hemoglobin chain synthesis resembling that of beta-thalassemia minor.
Defects in GATA1 are the cause of anemia without thrombocytopenia X-linked (XLAWT) [MIM:300835]. XLAWT is a form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals. -
Sequence similaritiesContains 2 GATA-type zinc fingers.
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DomainThe two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding.
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Post-translational
modificationsHighly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.
Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation by SUMO1 has no effect on transcriptional activity. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 2623 Human
- Entrez Gene: 14460 Mouse
- Entrez Gene: 25172 Rat
- Omim: 305371 Human
- SwissProt: P15976 Human
- SwissProt: P17679 Mouse
- SwissProt: P43429 Rat
- Unigene: 765 Human
see all -
Alternative names
- Anemia, X-linked, without thrombocytopenia, included antibody
- ERYF 1 antibody
- Eryf1 antibody
see all
Images
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Western blot - Anti-GATA1 antibody (ab40847)Anti-GATA1 antibody (ab40847) at 0.3 µg/ml + Human PBMC lysate (35µg protein in RIPA buffer).
Predicted band size: 43 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Protocols
Datasheets and documents
References
ab40847 has not yet been referenced specifically in any publications.
