Anti-GATA1 antibody (ab40847)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GATA1 antibody
See all GATA1 primary antibodies -
Description
Goat polyclonal to GATA1 -
Host species
Goat -
Tested applications
Suitable for: Flow Cyt, ICC/IF, ELISA, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Dog
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Immunogen
Synthetic peptide corresponding to Human GATA1 aa 65-76 (internal sequence).
Sequence:DAEAYRHSPVFQ
Database link: P15976 -
Positive control
- WB: K562 nuclear cell lysate and Human PBMC lysate ICC/IF: HeLa and NIH3T3 cells FC: K562 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab40847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use a concentration of 10 µg/ml. | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 43 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. -
Tissue specificity
Erythrocytes. -
Involvement in disease
Defects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.
Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050]; also knwon as thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. XLTT consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced hemoglobin chain synthesis resembling that of beta-thalassemia minor.
Defects in GATA1 are the cause of anemia without thrombocytopenia X-linked (XLAWT) [MIM:300835]. XLAWT is a form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals. -
Sequence similarities
Contains 2 GATA-type zinc fingers. -
Domain
The two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding. -
Post-translational
modificationsHighly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.
Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation by SUMO1 has no effect on transcriptional activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2623 Human
- Entrez Gene: 14460 Mouse
- Entrez Gene: 25172 Rat
- Omim: 305371 Human
- SwissProt: P15976 Human
- SwissProt: P17679 Mouse
- SwissProt: P43429 Rat
- Unigene: 765 Human
see all -
Alternative names
- Anemia, X-linked, without thrombocytopenia, included antibody
- ERYF 1 antibody
- Eryf1 antibody
see all
Images
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Western blot - Anti-GATA1 antibody (ab40847)All lanes : Anti-GATA1 antibody (ab40847) at 1 µg/ml
Lane 1 : K562 nuclear cell lysate
Lane 2 : Human Hippocampus lysate
Developed using the ECL technique.
Predicted band size: 43 kDaNegative Control: Human Hippocampus Lysate.
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Immunocytochemistry/ Immunofluorescence - Anti-GATA1 antibody (ab40847)Immunocytochemsitry/Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% Triton-permeabilized HeLa cells staining GATA1 with ab40847 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2ug/ml (green). DAPI was used as a nuclear counterstain (blue) and actin filaments were stained with phalloidin (red).
Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Flow Cytometry - Anti-GATA1 antibody (ab40847)Flow cytometric analysis of paraformaldehyde fixed, 0.15% triton-permeablized K562 cells labelling GATA1 with ab40847 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody (1ug/ml) (blue). Primary incubation carried out for 1hr.
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-GATA1 antibody (ab40847)Immunocytochemsitry/Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% Triton-permeabilized NIH3T3 cells staining GATA1 with ab40847 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2ug/ml (green). Primary incubation was carried out for 1 hour. DAPI was used as a nuclear counterstain (blue).
Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Western blot - Anti-GATA1 antibody (ab40847)Anti-GATA1 antibody (ab40847) at 0.3 µg/ml + Human PBMC lysate (35µg protein in RIPA buffer).
Predicted band size: 43 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Protocols
Datasheets and documents
References
ab40847 has not yet been referenced specifically in any publications.
