• Product name

    Anti-GATA1 antibody [EP2819Y]
    See all GATA1 primary antibodies
  • Description

    Rabbit monoclonal [EP2819Y] to GATA1
  • Host species

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GATA1 (N terminal). The exact sequence is proprietary.

  • Positive control

    • K562 cell lysate and permeabilized K562 cells
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.



Our Abpromise guarantee covers the use of ab76121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 43 kDa.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.
    • Tissue specificity

    • Involvement in disease

      Defects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.
      Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050]; also knwon as thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. XLTT consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced hemoglobin chain synthesis resembling that of beta-thalassemia minor.
      Defects in GATA1 are the cause of anemia without thrombocytopenia X-linked (XLAWT) [MIM:300835]. XLAWT is a form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals.
    • Sequence similarities

      Contains 2 GATA-type zinc fingers.
    • Domain

      The two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding.
    • Post-translational

      Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.
      Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation by SUMO1 has no effect on transcriptional activity.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • Anemia, X-linked, without thrombocytopenia, included antibody
      • ERYF 1 antibody
      • Eryf1 antibody
      • Erythroid transcription factor antibody
      • Erythrold transcription factor 1 antibody
      • GATA 1 antibody
      • GATA binding factor 1 antibody
      • GATA binding protein 1 (globin transcription factor 1) antibody
      • GATA binding protein 1 antibody
      • GATA-1 antibody
      • GATA-binding factor 1 antibody
      • GATA1 antibody
      • GATA1_HUMAN antibody
      • GF 1 antibody
      • GF-1 antibody
      • GF1 antibody
      • Globin transcription factor 1 antibody
      • NF E1 antibody
      • NF E1 DNA binding protein antibody
      • NF-E1 DNA-binding protein antibody
      • NFE 1 antibody
      • NFE1 antibody
      • Nuclear factor erythroid 1 antibody
      • Transcription factor GATA1 antibody
      • XLANP antibody
      • XLTDA antibody
      • XLTT antibody
      see all


    • Anti-GATA1 antibody [EP2819Y] (ab76121) at 1/2000 dilution + K562 cell lysate at 10 µg

      HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size: 43 kDa
      Observed band size: 43 kDa

    • Flow cytometric analysis of permeabilized K562 cells using ab76121 at a 1/50 dilution (red) or a rabbit IgG (negative) (green).


    ab76121 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As


    Thank you for contacting us. The antibody was testing using the protocol below. The rabbit monoclonal isotyope control is ab125938.

    1. Solutions and Reagents

    1.1. 1X PBS

    1.2. Blocking buffer: 0.5% BSA in 1X PBS

    1.3. 2% paraformaldehyde (1% solution - optional for storing samples)

    1.4. 1X FACS permeabilizing solution

    1.5. Fluorescently-conjugated secondary antibody (various forms)

    2. Protocol

    2.1. Collect 1x106 cells/sample.

    2.2. Wash cells once with blocking buffer.

    2.3. Fix cells with 2% paraformaldehyde and incubate at room temperature for 10 min.

    2.4. Wash cells once with blocking buffer.

    2.5. Add 0.5 ml 1X FACS permeabilizing solution and incubate at room temperature for 10 min.

    2.6. Wash cells once with blocking buffer.

    2.7. Incubate cells in blocking buffer for 30 min at room temperature.

    2.8. Add primary antibody 1/50 and incubate for 30 min at room temperature.

    2.9. Wash twice with blocking buffer and incubate with fluorescently-conjugated secondary antibody for 30 min at room temperature.

    2.10. Wash cells twice with blocking buffer.

    2.11. Re-suspend cells in 1X PBS and analyze on flow cytometry. Samples can be kept in 1% paraformaldehyde at 4 °C overnight.

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