RecombinantRabMAb

Recombinant Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)

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Immunoprecipitation - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)
  • ChIP - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)
  • Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17362] to GATA1 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, ChIP, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-GATA1 antibody [EPR17362] - BSA and Azide free
    See all GATA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17362] to GATA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, ChIP, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human GATA1 aa 1-200. The exact sequence is proprietary.
    Database link: P15976

  • Positive control

    • IHC-P: Human colon and cervix carcinoma tissues. ICC/IF: K562 cells. IP: K562 whole cell extract. ChIP: Chromatin from K562 cells.

  • General notes

    Ab241393 is the carrier-free version of ab181544. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab241393 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab241393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.

  • Tissue specificity

    Erythrocytes.

  • Involvement in disease

    Defects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.
    Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050]; also knwon as thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. XLTT consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced hemoglobin chain synthesis resembling that of beta-thalassemia minor.
    Defects in GATA1 are the cause of anemia without thrombocytopenia X-linked (XLAWT) [MIM:300835]. XLAWT is a form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals.

  • Sequence similarities

    Contains 2 GATA-type zinc fingers.

  • Domain

    The two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding.

  • Post-translational
    modifications

    Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.
    Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation by SUMO1 has no effect on transcriptional activity.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Anemia, X-linked, without thrombocytopenia, included antibody
      • ERYF 1 antibody
      • Eryf1 antibody
      • Erythroid transcription factor antibody
      • Erythrold transcription factor 1 antibody
      • GATA 1 antibody
      • GATA binding factor 1 antibody
      • GATA binding protein 1 (globin transcription factor 1) antibody
      • GATA binding protein 1 antibody
      • GATA-1 antibody
      • GATA-binding factor 1 antibody
      • GATA1 antibody
      • GATA1_HUMAN antibody
      • GF 1 antibody
      • GF-1 antibody
      • GF1 antibody
      • Globin transcription factor 1 antibody
      • NF E1 antibody
      • NF E1 DNA binding protein antibody
      • NF-E1 DNA-binding protein antibody
      • NFE 1 antibody
      • NFE1 antibody
      • Nuclear factor erythroid 1 antibody
      • Transcription factor GATA1 antibody
      • XLANP antibody
      • XLTDA antibody
      • XLTT antibody
      see all

    Images

    • Immunoprecipitation - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)
      Immunoprecipitation - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)

      GATA1 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab181544 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab181544 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Lane 1: K562 whole cell extract 10 µg (Input).
      Lane 2: ab181544 IP in K562 whole cell extract.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab181544 in K562 whole cell extract.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181544).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)

      Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling GATA1 with ab181544 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on leukocyte of Human cervical cancer is observed. Counterstained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181544).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling GATA1 with ab181544 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on leukocyte of Human colon stroma is observed. Counterstained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181544).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • ChIP - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)
      ChIP - Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)

      Chromatin was prepared from K562 (Human chronic myelogenous leukemia cells from bone marrow) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab181544 (blue), and 20µl of Anti rabbit IgG sepharose beads. 5μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

      “pro” stands for promoter region, while “NC2” stands for negative control which is negative loci at the promoter region.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181544).
    • Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)
      Anti-GATA1 antibody [EPR17362] - BSA and Azide free (ab241393)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab241393? Please let us know so that we can cite the reference in this datasheet.

    ab241393 has not yet been referenced specifically in any publications.

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