Overview

  • Product name
    Anti-GATA1 antibody - N-terminal
    See all GATA1 primary antibodies
  • Description
    Rabbit polyclonal to GATA1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human GATA1. N-terminal truncated Human recombinant protein
    Database link: P15976

  • Positive control
    • NCCIT, NTERRA and K562 cell lysates; U2OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab173816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 43 kDa.
ICC/IF 1/200.

Target

  • Function
    Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.
  • Tissue specificity
    Erythrocytes.
  • Involvement in disease
    Defects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.
    Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050]; also knwon as thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. XLTT consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced hemoglobin chain synthesis resembling that of beta-thalassemia minor.
    Defects in GATA1 are the cause of anemia without thrombocytopenia X-linked (XLAWT) [MIM:300835]. XLAWT is a form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals.
  • Sequence similarities
    Contains 2 GATA-type zinc fingers.
  • Domain
    The two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding.
  • Post-translational
    modifications
    Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.
    Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation by SUMO1 has no effect on transcriptional activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anemia, X-linked, without thrombocytopenia, included antibody
    • ERYF 1 antibody
    • Eryf1 antibody
    • Erythroid transcription factor antibody
    • Erythrold transcription factor 1 antibody
    • GATA 1 antibody
    • GATA binding factor 1 antibody
    • GATA binding protein 1 (globin transcription factor 1) antibody
    • GATA binding protein 1 antibody
    • GATA-1 antibody
    • GATA-binding factor 1 antibody
    • GATA1 antibody
    • GATA1_HUMAN antibody
    • GF 1 antibody
    • GF-1 antibody
    • GF1 antibody
    • Globin transcription factor 1 antibody
    • NF E1 antibody
    • NF E1 DNA binding protein antibody
    • NF-E1 DNA-binding protein antibody
    • NFE 1 antibody
    • NFE1 antibody
    • Nuclear factor erythroid 1 antibody
    • Transcription factor GATA1 antibody
    • XLANP antibody
    • XLTDA antibody
    • XLTT antibody
    see all

Images

  • Immunofluorescent analysis of formalin-fixed, permeabilized U2OS cells, labeling GATA1 with ab173816 at 1/200 dilution (green, middle panel) . Cells were washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Nuclei (blue, left panel) were stained with Hoechst 33342 dye.

  • All lanes : Anti-GATA1 antibody - N-terminal (ab173816) at 1/500 dilution

    Lane 1 : NCCIT whole cell lysate
    Lane 2 : NTERRA whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : goat anti-rabbit-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 43 kDa



    4-20% Tris-HCl polyacrylamide gel.

References

This product has been referenced in:
  • Gastou M  et al. The severe phenotype of Diamond-Blackfan anemia is modulated by heat shock protein 70. Blood Adv 1:1959-1976 (2017). Read more (PubMed: 29296843) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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