Anti-GATA2 antibody (ab153820)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-GATA2 antibody
See all GATA2 primary antibodies -
DescriptionRabbit polyclonal to GATA2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Pig
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 130-397 of Human GATA2.
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Positive control
- A549, HeLa, HepG2, HCT116 and H1299 cell lysates; HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab153820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 50 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionTranscriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.
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Tissue specificityEndothelial cells.
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Sequence similaritiesContains 2 GATA-type zinc fingers.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 2624 Human
- Omim: 137295 Human
- SwissProt: P23769 Human
- Unigene: 367725 Human
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Alternative names
- DCML antibody
- Endothelial transcription factor GATA 2 antibody
- Endothelial transcription factor GATA-2 antibody
see all
Images
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Western blot - Anti-GATA2 antibody (ab153820)Anti-GATA2 antibody (ab153820) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-GATA2 antibody (ab153820)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling GATA2 with ab153820 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
References
This product has been referenced in:
- Diaferia GR et al. Dissection of transcriptional and cis-regulatory control of differentiation in human pancreatic cancer. EMBO J 35:595-617 (2016). IHC-P ; Human . Read more (PubMed: 26769127) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"