Anti-GATA2 antibody (ab153820)

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Western blot - Anti-GATA2 antibody (ab153820)
  • Immunocytochemistry/ Immunofluorescence - Anti-GATA2 antibody (ab153820)

Key features and details

  • Rabbit polyclonal to GATA2
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-GATA2 antibody
    See all GATA2 primary antibodies

  • Description

    Rabbit polyclonal to GATA2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 130-397 of Human GATA2.

  • Positive control

    • A549, HeLa, HepG2, HCT116 and H1299 cell lysates; HeLa cells.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab153820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Pig
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.

  • Tissue specificity

    Endothelial cells.

  • Sequence similarities

    Contains 2 GATA-type zinc fingers.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • DCML antibody
      • Endothelial transcription factor GATA 2 antibody
      • Endothelial transcription factor GATA-2 antibody
      • Endothelial transcription factor GATA2 antibody
      • FLJ45948 antibody
      • GATA 2 antibody
      • GATA binding protein 2 antibody
      • GATA-binding protein 2 antibody
      • Gata2 antibody
      • GATA2_HUMAN antibody
      • IMD21 antibody
      • MGC2306 antibody
      • MONOMAC antibody
      • NFE 1B antibody
      • NFE1B antibody
      • OTTHUMP00000216240 antibody
      see all

    Images

    • Western blot - Anti-GATA2 antibody (ab153820)
      Western blot - Anti-GATA2 antibody (ab153820)
      Anti-GATA2 antibody (ab153820) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

      Predicted band size: 50 kDa



      10% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-GATA2 antibody (ab153820)
      Immunocytochemistry/ Immunofluorescence - Anti-GATA2 antibody (ab153820)
      Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling GATA2 with ab153820 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

    References (1)

    Publishing research using ab153820? Please let us know so that we can cite the reference in this datasheet.

    ab153820 has been referenced in 1 publication.

    • Diaferia GR  et al. Dissection of transcriptional and cis-regulatory control of differentiation in human pancreatic cancer. EMBO J 35:595-617 (2016). IHC-P ; Human . PubMed: 26769127

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