Anti-GATA2 antibody (ab153820)
Rabbit polyclonal GATA2 antibody. Validated in WB, ICC/IF and tested in Human. Cited in 1 publication(s). Immunogen corresponding to recombinant fragment.
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Anti-GATA2 antibody
See all GATA2 primary antibodies -
Description
Rabbit polyclonal to GATA2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Pig
-
Immunogen
Recombinant fragment, corresponding to a region within amino acids 130-397 of Human GATA2.
-
Positive control
- A549, HeLa, HepG2, HCT116 and H1299 cell lysates; HeLa cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab153820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 50 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
-
Function
Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'. -
Tissue specificity
Endothelial cells. -
Sequence similarities
Contains 2 GATA-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 2624 Human
- Omim: 137295 Human
- SwissProt: P23769 Human
- Unigene: 367725 Human
-
Alternative names
- DCML antibody
- Endothelial transcription factor GATA 2 antibody
- Endothelial transcription factor GATA-2 antibody
see all
Images
-
Western blot - Anti-GATA2 antibody (ab153820)Anti-GATA2 antibody (ab153820) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-GATA2 antibody (ab153820)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling GATA2 with ab153820 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
References
This product has been referenced in:
- Diaferia GR et al. Dissection of transcriptional and cis-regulatory control of differentiation in human pancreatic cancer. EMBO J 35:595-617 (2016). IHC-P ; Human . Read more (PubMed: 26769127) »
