Anti-GATA2 antibody (ab153820)
Key features and details
- Rabbit polyclonal to GATA2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GATA2 antibody
See all GATA2 primary antibodies -
Description
Rabbit polyclonal to GATA2 -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanWB Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 130-397 of Human GATA2.
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Positive control
- A549, HeLa, HepG2, HCT116 and H1299 cell lysates; HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
|
WB |
Human
|
All applications |
Pig
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Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
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|
ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'. -
Tissue specificity
Endothelial cells. -
Sequence similarities
Contains 2 GATA-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2624 Human
- Omim: 137295 Human
- SwissProt: P23769 Human
- Unigene: 367725 Human
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Alternative names
- DCML antibody
- Endothelial transcription factor GATA 2 antibody
- Endothelial transcription factor GATA-2 antibody
see all
Images
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Anti-GATA2 antibody (ab153820) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling GATA2 with ab153820 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Protocols
References (1)
ab153820 has been referenced in 1 publication.
- Diaferia GR et al. Dissection of transcriptional and cis-regulatory control of differentiation in human pancreatic cancer. EMBO J 35:595-617 (2016). IHC-P ; Human . PubMed: 26769127