Overview

  • Product name
    Anti-GATA2 antibody - N-terminal
    See all GATA2 primary antibodies
  • Description
    Rabbit polyclonal to GATA2 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human GATA2. N-terminal truncated Human recombinant protein
    Database link: P23769

  • Positive control
    • NTERRA and mouse MEF cell lysates; Human prostate tissue; HeLa and U251 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab173817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 51 kDa.
ICC/IF 1/20 - 1/200.
IHC-P 1/100 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'.
  • Tissue specificity
    Endothelial cells.
  • Sequence similarities
    Contains 2 GATA-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DCML antibody
    • Endothelial transcription factor GATA 2 antibody
    • Endothelial transcription factor GATA-2 antibody
    • Endothelial transcription factor GATA2 antibody
    • FLJ45948 antibody
    • GATA 2 antibody
    • GATA binding protein 2 antibody
    • GATA-binding protein 2 antibody
    • Gata2 antibody
    • GATA2_HUMAN antibody
    • IMD21 antibody
    • MGC2306 antibody
    • MONOMAC antibody
    • NFE 1B antibody
    • NFE1B antibody
    • OTTHUMP00000216240 antibody
    see all

Images

  • Immunofluorescent analysis of formalin-fixed, permeabilized HeLa cells, labeling GATA2 with ab173817 at 1/200 dilution (green). Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.

  • Immunohistochemical analysis of Human prostate tissue, labeling GATA2 with ab173817 at 1/100 dilution (right image). Detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin.

  • Immunofluorescent analysis of formalin-fixed, permeabilized U251 cells, labeling GATA2 with ab173817 at 1/20 dilution (green). Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.

  • Immunofluorescent analysis of formalin-fixed, permeabilized Human cells, labeling GATA2 with ab173817 at 1/100 dilution (green). Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.

  • Immunofluorescent analysis of formalin-fixed, permeabilized HeLa cells, labeling GATA2 with ab173817 at 1/200 dilution (green). Cells were washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Nuclei (blue) were stained with Hoechst 33342 dye.

  • All lanes : Anti-GATA2 antibody - N-terminal (ab173817) at 1/500 dilution

    Lane 1 : mouse MEF cell lysate
    Lane 2 : NTERRA cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : goat anti-rabbit-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 51 kDa

References

This product has been referenced in:
  • Qiu C  et al. The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis. Nat Commun 8:15426 (2017). IF ; Mouse . Read more (PubMed: 28569748) »
See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spinal Cord)
Permeabilization
Yes - 0.3% Triton-PBS
Specification
Spinal Cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Sep 12 2017

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