Immunohistochemistry (Frozen sections) abreview for Anti-GATA2 antibody - N-terminal

Poor
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spinal Cord)
Permeabilization
Yes - 0.3% Triton-PBS
Specification
Spinal Cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% NGS in 0.3% PBS-T
Dilution
1/500

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: Alexa Fluor goat anti-rabbit 546
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 546

Additional data

Additional Notes
Non-specific binding in all cells within tissue despite multiple rounds of optimization and changes in dilution factors and reagents. In image: Blue is DAPI, red is Gata2

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Submitted Sep 12 2017

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