Recombinant
RabMAb

Recombinant Anti-GATA3 antibody [EPR16651] (Alexa Fluor® 568) (ab208897)

Overview

  • Product name

    Anti-GATA3 antibody [EPR16651] (Alexa Fluor® 568)
    See all GATA3 primary antibodies
  • Description

    Rabbit monoclonal [EPR16651] to GATA3 (Alexa Fluor® 568)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 568. Ex: 578nm, Em: 603nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human GATA3 aa 1-200. The exact sequence is proprietary.
    Database link: P23771

  • Positive control

    • ICC/IF: T47D cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab208897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/625.

This product gave a positive signal in T47D cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function

    Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
  • Tissue specificity

    T-cells and endothelial cells.
  • Involvement in disease

    Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
  • Sequence similarities

    Contains 2 GATA-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Form

    There are 2 isoforms produced by alternative splicing.
  • Alternative names

    • GATA 3 antibody
    • GATA binding factor 3 antibody
    • GATA binding protein 3 antibody
    • GATA-binding factor 3 antibody
    • Gata3 antibody
    • GATA3_HUMAN antibody
    • HDR antibody
    • HDRS antibody
    • MGC2346 antibody
    • MGC5199 antibody
    • MGC5445 antibody
    • Trans acting T cell specific transcription factor GATA 3 antibody
    • Trans-acting T-cell-specific transcription factor GATA-3 antibody
    see all

Images

  • ab208897 staining GATA3 in T47D cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208897 at 1/625 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

     

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

     

    This product also gave a positive signal under the same testing conditions in T47D cells fixed with 100% methanol (5 min)

References

ab208897 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab208897.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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