Product nameAnti-GATA3 antibody [EPR16651] (Alexa Fluor® 647)
See all GATA3 primary antibodies
DescriptionRabbit monoclonal [EPR16651] to GATA3 (Alexa Fluor® 647)
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human GATA3 aa 1-200. The exact sequence is proprietary.
Database link: P23771
- ICC/IF: T47D cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
- Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
- Anti-GATA3 antibody [EPR16651] (Alexa Fluor® 488) (ab208895)
- Anti-GATA3 antibody [EPR16651] (Alexa Fluor® 568) (ab208897)
- Anti-GATA3 antibody [EPR16651] (Alexa Fluor® 555) (ab210672)
- Anti-GATA3 antibody [EPR16651] - BSA and Azide free (ab214804)
- Anti-GATA3 antibody [EPR16651] (Allophycocyanin) (ab225418)
- Anti-GATA3 antibody [EPR16651] (Phycoerythrin) (ab225419)
Our Abpromise guarantee covers the use of ab208896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in T47D cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
FunctionTranscriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
Tissue specificityT-cells and endothelial cells.
Involvement in diseaseDefects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
Sequence similaritiesContains 2 GATA-type zinc fingers.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing.
- GATA 3 antibody
- GATA binding factor 3 antibody
- GATA binding protein 3 antibody
ab208896 staining GATA3 in T47D cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208896 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in T47D cells fixed with 100% methanol (5 min).
ab208896 has not yet been referenced specifically in any publications.