Product nameAnti-GATA3 antibody [EPR16651] (Allophycocyanin)
See all GATA3 primary antibodies
DescriptionRabbit monoclonal [EPR16651] to GATA3 (Allophycocyanin)
ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Recombinant fragment within Human GATA3 aa 1-200. The exact sequence is proprietary.
Database link: P23771
- Flow Cyt: MCF7 cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428)
- Anti-GATA3 antibody [EPR16651] (Alexa Fluor® 488) (ab208895)
- Anti-GATA3 antibody [EPR16651] (Alexa Fluor® 647) (ab208896)
- Anti-GATA3 antibody [EPR16651] (Alexa Fluor® 568) (ab208897)
- Anti-GATA3 antibody [EPR16651] (Alexa Fluor® 555) (ab210672)
- Anti-GATA3 antibody [EPR16651] - BSA and Azide free (ab214804)
- Anti-GATA3 antibody [EPR16651] (Phycoerythrin) (ab225419)
Our Abpromise guarantee covers the use of ab225418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cellular localisation of this product has been verified in ICC/IF.
FunctionTranscriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
Tissue specificityT-cells and endothelial cells.
Involvement in diseaseDefects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
Sequence similaritiesContains 2 GATA-type zinc fingers.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing.
- GATA 3 antibody
- GATA binding factor 3 antibody
- GATA binding protein 3 antibody
Overlay histogram showing MCF7 cells stained with ab225418 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225418, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
This antibody gave a positive signal in MCF7 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
ab225418 has not yet been referenced specifically in any publications.