Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Kidney H9 Organoids)
Permeabilization
Yes - 0.05% tween 20
Specification
Kidney H9 Organoids
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea Block
Dilution
1/200
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
The GATA3 nuclear signal is pseudo colored in Green and the negative nuclei are in Blue using Hoechst .
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Feb 06 2020