Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9 Tris EDTA
Permeabilization
No
Specification
Lymph node
Blocking step
Zytomed Systems Antibody Diluent as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: Zytomed Systems Antibody Diluent
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Zytomed Systems HRP Polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Heat mediated antigen retrieval was performed using a inverter microwave with 3 min @ 1000W and 30 min @ 100W
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Submitted Jun 11 2020