Application
Immunocytochemistry
Sample
Human Cultured Cells (Embryonic stem cells)
Permeabilization
Yes - methanol
Specification
Embryonic stem cells
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10%FBS in PBS
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used:
Additional data
Additional Notes
Fantastic!
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Dec 21 2021