Key features and details
- Mouse monoclonal [mAbcam77073] to GATA3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GATA3 antibody [mAbcam77073]
See all GATA3 primary antibodies
DescriptionMouse monoclonal [mAbcam77073] to GATA3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig
Synthetic peptide corresponding to Human GATA3 aa 1-100 (N terminal).
(Peptide available as
- This antibody gave a positive signal in the following human tissue lysates: Thymus; Lymph Node; Spleen; Tonsil.
This antibody clone is manufactured by Abcam.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab77073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).|
FunctionTranscriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
Tissue specificityT-cells and endothelial cells.
Involvement in diseaseDefects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
Sequence similaritiesContains 2 GATA-type zinc fingers.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing.
- GATA 3 antibody
- GATA binding factor 3 antibody
- GATA binding protein 3 antibody
All lanes : Anti-GATA3 antibody [mAbcam77073] (ab77073) at 5 µg/ml
Lane 1 : Human thymus tissue lysate - total protein (ab30146)
Lane 2 : Human lymph node tissue lysate - total protein (ab29871)
Lane 3 : Human spleen tissue lysate - total protein (ab29699)
Lane 4 : Tonsil (Human) Tissue Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The binding pattern of ab77073 in human thymus, lymph node, spleen and tonsil tissue lysates is similar to that of the polyclonal antibody to the same target (ab32858) in MOLT4, Jurkat, THP1 and Ramos whole cell lysates.
ab77073 has not yet been referenced specifically in any publications.