Overview

  • Product name

    Anti-GATA3 antibody [mAbcam77073]
    See all GATA3 primary antibodies
  • Description

    Mouse monoclonal [mAbcam77073] to GATA3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to Human GATA3 aa 1-100 (N terminal).
    (Peptide available as ab107920)

  • Positive control

    • This antibody gave a positive signal in the following human tissue lysates: Thymus; Lymph Node; Spleen; Tonsil.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab77073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).

Target

Images

  • All lanes : Anti-GATA3 antibody [mAbcam77073] (ab77073) at 5 µg/ml

    Lane 1 : Human thymus tissue lysate - total protein (ab30146)
    Lane 2 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)
    Lane 4 : Tonsil (Human) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes


    The binding pattern of ab77073 in human thymus, lymph node, spleen and tonsil tissue lysates is similar to that of the polyclonal antibody to the same target (ab32858) in MOLT4, Jurkat, THP1 and Ramos whole cell lysates.

References

ab77073 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab77073.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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