Product nameGATA3 overexpression 293T lysate (whole cell)
ab94325 is a 293T cell transfected lysate in which Human GATA3 has been transiently over-expressed using a pCMV-GATA3 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundDisease: Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome. Function: Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Similarity: Contains 2 GATA-type zinc fingers. Tissue specificity: T-cells and endothelial cells.
Our Abpromise guarantee covers the use of ab94325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94325 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-GATA3 antibody (ab89776) at 1/500 dilution
Lane 1 :
GATA3 overexpression 293T lysate (whole cell) (ab94325)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94325 has not yet been referenced specifically in any publications.