Product nameAnti-GATA4 antibody
See all GATA4 primary antibodies
DescriptionRabbit polyclonal to GATA4
Tested applicationsSuitable for: IHC-P, ICC/IF, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 392-442 of Human GATA4 (NP_002043.2).
- 293T whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab124265 was affinity purified using an epitope specific to GATA4 immobilized on solid support
Our Abpromise guarantee covers the use of ab124265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 44 kDa.|
|IP||Use a concentration of 2 - 10 µg/ml.|
FunctionTranscriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
Involvement in diseaseAtrial septal defect 2
Ventricular septal defect 1
Tetralogy of Fallot
Atrioventricular septal defect 4
Testicular anomalies with or without congenital heart disease
GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesContains 2 GATA-type zinc fingers.
modificationsMethylation at Lys-300 attenuates transcriptional activity.
- Information by UniProt
- ASD2 antibody
- GATA 4 antibody
- GATA binding protein 4 antibody
ab124265 staining GATA4 in human pluripotent stem cell derived cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 5% serum for 15 minutes at room temperature. Samples were incubated with primary antibody (1/800) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG monoclonal (1/1000) was used as the secondary antibody.
Nuclear specific staining of GATA4 (yellow) in GFP positive cardiomyocytes (green), with GFP driven by a cardiac specific promoter.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach linitis plastica tissue labelling GATA4 with ab124265 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-GATA4 antibody (ab124265) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg/ml
Lane 2 : 293T whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 50 µg/ml
Lane 4 : Jurkat whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 3 minutes
ab124265 at 1 µg/ml detecting GATA4 in 293T whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of GATA4
Lane 2: ab124265 at 6 µg/mg of lysate
Lane 3: Control IgG
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds