Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
Involvement in disease
Atrial septal defect 2 Ventricular septal defect 1 Tetralogy of Fallot Atrioventricular septal defect 4 Testicular anomalies with or without congenital heart disease GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Contains 2 GATA-type zinc fingers.
Methylation at Lys-300 attenuates transcriptional activity.
ab124265 at 1 µg/ml detecting GATA4 in 293T whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of GATA4
Lane 2: ab124265 at 6 µg/mg of lysate
Lane 3: Control IgG
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds
Immunocytochemistry/ Immunofluorescence - Anti-GATA4 antibody (ab124265)This image is courtesy of an anonymous Abreview
ab124265 staining GATA4 in human pluripotent stem cell derived cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 5% serum for 15 minutes at room temperature. Samples were incubated with primary antibody (1/800) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG monoclonal (1/1000) was used as the secondary antibody.
Nuclear specific staining of GATA4 (yellow) in GFP positive cardiomyocytes (green), with GFP driven by a cardiac specific promoter.