Key features and details
- Rabbit polyclonal to GATA4
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GATA4 antibody
See all GATA4 primary antibodies
DescriptionRabbit polyclonal to GATA4
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 392-442 of Human GATA4 (NP_002043.2).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab124265 was affinity purified using an epitope specific to GATA4 immobilized on solid support
Our Abpromise guarantee covers the use of ab124265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 44 kDa.|
|IP||Use a concentration of 2 - 10 µg/ml.|
FunctionTranscriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
Involvement in diseaseAtrial septal defect 2
Ventricular septal defect 1
Tetralogy of Fallot
Atrioventricular septal defect 4
Testicular anomalies with or without congenital heart disease
GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesContains 2 GATA-type zinc fingers.
modificationsMethylation at Lys-300 attenuates transcriptional activity.
- Information by UniProt
- ASD2 antibody
- GATA 4 antibody
- GATA binding protein 4 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach linitis plastica tissue labelling GATA4 with ab124265 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-GATA4 antibody (ab124265) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg/ml
Lane 2 : 293T whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 50 µg/ml
Lane 4 : Jurkat whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 3 minutes
ab124265 at 1 µg/ml detecting GATA4 in 293T whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of GATA4
Lane 2: ab124265 at 6 µg/mg of lysate
Lane 3: Control IgG
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds
ab124265 has been referenced in 3 publications.
- Fang T et al. Functional analysis of the congenital heart disease-associated GATA4 H436Y mutation in vitro. Mol Med Rep 20:2325-2331 (2019). PubMed: 31322241
- Han Q et al. GATA4 is highly expressed in childhood acute lymphoblastic leukemia, promotes cell proliferation and inhibits apoptosis by activating BCL2 and MDM2. Mol Med Rep 16:6290-6298 (2017). PubMed: 28849107
- Enane FO et al. GATA4 loss of function in liver cancer impedes precursor to hepatocyte transition. J Clin Invest 127:3527-3542 (2017). PubMed: 28758902