Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GATA4 antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Testis
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 30 minute(s) · Temperature: 20°C
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti rabiit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
To be able to see the good signal I had to maximize the concentration of antibody up to 1:100. At 1:200, I can still see the signal but 1:100 is better.

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Submitted Aug 30 2011

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