Western blot abreview for Anti-GATA4 antibody

Excellent
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12)
Sample
Mouse Cell lysate - nuclear (Gata4 over-expressing undifferentiated mouse ES ce)
Specification
Gata4 over-expressing undifferentiated mouse ES ce
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
1 hour(s) and 15 minute(s) · Temperature: 18°C · Diluent: 5% Milk
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL Prime
Bands
Specific: 50 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
Undifferentiate WT mouse ES cells
Positive control
Day 4 differentiated WT mouse ES cells

Ms. Elisa Ponsele

Verified customer

Submitted Dec 20 2013

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