Western blot abreview for Anti-GATA4 antibody

Good
Abreviews
Application
Western blot
Sample
Pig Tissue lysate - whole (Total heart)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Total heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBS-0,2% Tween 20
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 44 kDa Non-specific: 55, 110 (faint) kDa
Positive control
Lane1: Adult mouse heart tissue lysate Lane2: Adult pig total heart tissue lysate

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Submitted Sep 13 2017

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