Product nameAnti-GATA4 antibody [EPR4768]
See all GATA4 primary antibodies
DescriptionRabbit monoclonal [EPR4768] to GATA4
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human GATA4 aa 1-100 (N terminal).
Database link: P43694
- HepG2, Caco-2 and HeLa whole cell lysate (ab150035); HepG2 cells.
This antibody was developed as part of a collaboration between Dartmouth College and the lab of Sergei Tevosian.
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.00 x 10 -12 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
- Anti-GATA4 antibody [EPR4768] (Alexa Fluor® 488) (ab194071)
- Anti-GATA4 antibody [EPR4768] (Alexa Fluor® 647) (ab194072)
- Anti-GATA4 antibody [EPR4768] (HRP) (ab194073)
- Anti-GATA4 antibody [EPR4768] (Alexa Fluor® 555) (ab202520)
- Anti-GATA4 antibody [EPR4768] (Alexa Fluor® 594) (ab202521)
- Anti-GATA4 antibody [EPR4768] - BSA and Azide free (ab219725)
Our Abpromise guarantee covers the use of ab134057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 54 kDa.|
For unpurified use at 1/1200.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
For unpurified use at 1/100 - 1/250.
FunctionTranscriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
Involvement in diseaseAtrial septal defect 2
Ventricular septal defect 1
Tetralogy of Fallot
Atrioventricular septal defect 4
Testicular anomalies with or without congenital heart disease
GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesContains 2 GATA-type zinc fingers.
modificationsMethylation at Lys-300 attenuates transcriptional activity.
- Information by UniProt
- ASD2 antibody
- GATA 4 antibody
- GATA binding protein 4 antibody
ab134057 staining GATA4 in the PC-3 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150). An Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.
Overlay histogram showing Caco-2 cells stained with ab134057 (red line) at 1/600 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.
Anti-GATA4 antibody [EPR4768] (ab134057) at 1/2000 dilution + Mouse spleen lysate at 10 µg
Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Predicted band size: 54 kDa
Anti-GATA4 antibody [EPR4768] (ab134057) at 1/2000 dilution + HepG2 lysate at 10 µg
Goat Anti-Rabbit IgG, (H+L), HRP-conjugated
Predicted band size: 54 kDa
Overlay histogram showing HeLa cells stained with ab134057, unpurified (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134057, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
All lanes : Anti-GATA4 antibody [EPR4768] (ab134057) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : Caco-2 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 54 kDa
Immunofluorescent analysis of HepG2 cells labelling GATA4 with ab134057, unpuified, at 1/100 dilution.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Wu L et al. Bone morphogenetic protein 4 promotes the differentiation of Tbx18-positive epicardial progenitor cells to pacemaker-like cells. Exp Ther Med 17:2648-2656 (2019). Read more (PubMed: 30906456) »
- Huang H et al. CRISPR/dCas9-mediated activation of multiple endogenous target genes directly converts human foreskin fibroblasts into Leydig-like cells. J Cell Mol Med 23:6072-6084 (2019). Read more (PubMed: 31264792) »