Product nameAnti-GATA4 antibody [EPR4768] (HRP)
See all GATA4 primary antibodies
DescriptionRabbit monoclonal [EPR4768] to GATA4 (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human GATA4 (N terminal). The exact sequence is proprietary.
- WB: HepG2 whole cell lysates.
This antibody was developed as part of a collaboration between Dartmouth College and the lab of Sergei Tevosian.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Dissociation constant (KD)KD = 2.00 x 10 -12 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-GATA4 antibody [EPR4768] (ab134057)
- Anti-GATA4 antibody [EPR4768] (Alexa Fluor® 488) (ab194071)
- Anti-GATA4 antibody [EPR4768] (Alexa Fluor® 647) (ab194072)
- Anti-GATA4 antibody [EPR4768] (Alexa Fluor® 555) (ab202520)
- Anti-GATA4 antibody [EPR4768] (Alexa Fluor® 594) (ab202521)
- Anti-GATA4 antibody [EPR4768] - BSA and Azide free (ab219725)
Our Abpromise guarantee covers the use of ab194073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).|
FunctionTranscriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
Involvement in diseaseAtrial septal defect 2
Ventricular septal defect 1
Tetralogy of Fallot
Atrioventricular septal defect 4
Testicular anomalies with or without congenital heart disease
GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesContains 2 GATA-type zinc fingers.
modificationsMethylation at Lys-300 attenuates transcriptional activity.
- Information by UniProt
- ASD2 antibody
- GATA 4 antibody
- GATA binding protein 4 antibody
Anti-GATA4 antibody [EPR4768] (HRP) (ab194073) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab194073 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab194073 has not yet been referenced specifically in any publications.