Product nameAnti-GATA5 antibody
DescriptionRabbit polyclonal to GATA5
SpecificityThis antibody recognizes GATA5
Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
- SP2/0 lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab48820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionBinds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer. May play an important role in the transcriptional program(s) that underlies smooth muscle cell diversity.
Sequence similaritiesContains 2 GATA-type zinc fingers.
- Information by UniProt
- GATA binding protein 5 antibody
- GATA-binding factor 5 antibody
- GATA5 antibody
Anti-GATA5 antibody (ab48820) at 1.25 µg/ml + SP2/0 lysate at 10 µg with 5% skimmed milk in PBS.
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 44 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
Gel concentration 12%
ab48820 staining GATA5 in murine epicardial explant culture by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in formaldehyde, permeabilized using 0.5% Triton X-100 in PBS, blocked with 1% goat serum 10% BSA 0.1% Triton X-100 PBS and then incubated with ab48820 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-rabbit IgG (H+L) used at a 1/200 dilution.
This product has been referenced in:
- Roe JS et al. Enhancer Reprogramming Promotes Pancreatic Cancer Metastasis. Cell 170:875-888.e20 (2017). Read more (PubMed: 28757253) »
- Risebro CA et al. Characterisation of the human embryonic and foetal epicardium during heart development. Development 142:3630-6 (2015). IHC ; Human . Read more (PubMed: 26395486) »