Key features and details
- Rabbit polyclonal to Gata6
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Gata6 antibody
See all Gata6 primary antibodies
DescriptionRabbit polyclonal to Gata6
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
- HeLa cells; Human stomach tissue; 293T, MCF7, K562 and 3T3L1 whole cell lysates.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab175349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 60 kDa.|
FunctionThought to be important for regulating terminal differentiation and/or proliferation.
Tissue specificityExpressed in heart, gut and gut-derived tissues.
Sequence similaritiesContains 2 GATA-type zinc fingers.
DomainThe GATA-type zinc fingers mediate interaction with LMCD1.
- Information by UniProt
- Gata binding factor 6 antibody
- Gata binding protein 6 antibody
- GATA-binding factor 6 antibody
All lanes : Anti-Gata6 antibody (ab175349) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : 3T3L1 cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat anti-rabbit-HRP at 1/15000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa
Immunohistochemical analysis of Human stomach tissue labeling Gata6 with ab175349 at 1/100 dilution. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Following labeling, tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.
Immunofluorescent analysis of HeLa cells labeling Gata6 with ab175349 at 1/200 dilution (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Following detection with the primary antibody, the cells were then washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1/200 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.
ab175349 has been referenced in 2 publications.
- Liu J et al. Gata4 regulates hedgehog signaling and Gata6 expression for outflow tract development. PLoS Genet 15:e1007711 (2019). PubMed: 31120883
- Ye B et al. LncKdm2b controls self-renewal of embryonic stem cells via activating expression of transcription factor Zbtb3. EMBO J 37:N/A (2018). PubMed: 29535137