Overview

  • Product name

  • Description

    Rabbit polyclonal to Gata6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Gata6 aa 561-579. Plus synthetic peptide corresponding to Human Gata6 aa 12-31 (KRFGAAGADASDSRAFPARE)
    Sequence:

    KYSGQDGLYIGVSLASPAE


    Database link: Q92908

  • Positive control

    • HeLa cells; Human stomach tissue; 293T, MCF7, K562 and 3T3L1 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab175349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 60 kDa.

Target

  • Function

    Thought to be important for regulating terminal differentiation and/or proliferation.
  • Tissue specificity

    Expressed in heart, gut and gut-derived tissues.
  • Sequence similarities

    Contains 2 GATA-type zinc fingers.
  • Domain

    The GATA-type zinc fingers mediate interaction with LMCD1.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Gata binding factor 6 antibody
    • Gata binding protein 6 antibody
    • GATA-binding factor 6 antibody
    • Gata6 antibody
    • GATA6_HUMAN antibody
    • Transcription factor Gata 6 antibody
    • Transcription factor GATA-6 antibody
    see all

Images

  • All lanes : Anti-Gata6 antibody (ab175349) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : 3T3L1 cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit-HRP at 1/15000 dilution

    Developed using the ECL technique.

    Predicted band size: 60 kDa

  • Immunohistochemical analysis of Human stomach tissue labeling Gata6 with ab175349 at 1/100 dilution. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature.  Following labeling, tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunofluorescent analysis of HeLa cells labeling Gata6 with ab175349 at 1/200 dilution (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Following detection with the primary antibody, the cells were then washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1/200 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.

References

ab175349 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175349.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up