Overview

  • Product name

  • Description

    Rabbit polyclonal to GATAD2B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human GATAD2B aa 1-50 (N terminal).
    Database link: NP_065750.1

  • Positive control

    • HeLa and 293T whole cell lysates.
  • General notes

     This product was previously labelled as p66 beta

     

Properties

Applications

Our Abpromise guarantee covers the use of ab76924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Has transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 1 GATA-type zinc finger.
  • Domain

    CR1 is required for interaction with MBD3 and transcription repression.
    CR2 is required for localization to discrete loci in the nucleus.
  • Cellular localization

    Nucleus. Nuclear, in discrete foci.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ37346 antibody
    • GATA zinc finger domain containing 2B antibody
    • GATA zinc finger domain containing protein 2B antibody
    • GATA zinc finger domain-containing protein 2B antibody
    • GATAD2B antibody
    • KIAA1150 antibody
    • MGC138257 antibody
    • MGC138285 antibody
    • mKIAA1150 antibody
    • p66/p68 antibody
    • P66B_HUMAN antibody
    • P66beta antibody
    • RGD1308533 antibody
    • transcription repressor p66 beta component of the MeCP1 complex antibody
    • Transcriptional repressor p66 beta antibody
    • Transcriptional repressor p66-beta antibody
    see all

Images

  • All lanes : Anti-GATAD2B antibody (ab76924) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 66 kDa
    Observed band size: 71 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • Detection of GATAD2B by Western Blot of Immunprecipitate.
    ab76924 at 1µg/ml staining GATAD2B in HeLa whole cell lysate immunoprecipitated using ab76924 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

This product has been referenced in:

  • Chalei V  et al. The long non-coding RNA Dali is an epigenetic regulator of neural differentiation. Elife 3:e04530 (2014). Read more (PubMed: 25415054) »
  • Cui S  et al. Nuclear Receptors TR2 and TR4 Recruit Multiple Epigenetic Transcriptional Corepressors That Associate Specifically with the Embryonic {beta}-Type Globin Promoters in Differentiated Adult Erythroid Cells. Mol Cell Biol 31:3298-311 (2011). Read more (PubMed: 21670149) »
See all 2 Publications for this product

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