Key features and details
- Rabbit polyclonal to GATAD2B
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-GATAD2B antibody
See all GATAD2B primary antibodies
DescriptionRabbit polyclonal to GATAD2B
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster
Synthetic peptide corresponding to Human GATAD2B aa 50-100.
Database link: NP_065750.1
- HeLa and 293T whole cell lysates.
This product was previously labelled as p66 beta
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab76925 was affinity purified using an epitope specific to GATAD2B immobilized on solid support.
Our Abpromise guarantee covers the use of ab76925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 66 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionHas transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus.
Sequence similaritiesContains 1 GATA-type zinc finger.
DomainCR1 is required for interaction with MBD3 and transcription repression.
CR2 is required for localization to discrete loci in the nucleus.
Cellular localizationNucleus. Nuclear, in discrete foci.
- Information by UniProt
- FLJ37346 antibody
- GATA zinc finger domain containing 2B antibody
- GATA zinc finger domain containing protein 2B antibody
All lanes : Anti-GATAD2B antibody (ab76925) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 66 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling GATAD2B with ab76925 at 1/200 (1µg/ml). Detection: DAB.
ab76925, at 1µg/ml, staining GATAD2B in HeLa whole cell lysate immunoprecipitated using ab76925 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
ab76925 has not yet been referenced specifically in any publications.