Overview

  • Product name

    Anti-GATM antibody [OTI1C9]
    See all GATM primary antibodies
  • Description

    Mouse monoclonal [OTI1C9] to GATM
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human GATM. Produced in HEK293 cells (NP_001473).
    Database link: P50440

  • Positive control

    • WB: HEK-293T transfected lysate. IHC-P: Human kidney, liver, liver carcinoma or pancreas tissue Flow: HEK-293T cells. ICC/IF: COS-7 transfected cells

Properties

Applications

Our Abpromise guarantee covers the use of ab119269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 48 kDa.
IHC-P 1/150.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100.

Target

  • Function

    Catalyzes the biosynthesis of guanidinoacetate, the immediate precursor of creatine. Creatine plays a vital role in energy metabolism in muscle tissues. May play a role in embryonic and central nervous system development. May be involved in the response to heart failure by elevating local creatine synthesis.
  • Tissue specificity

    Expressed in brain, heart, kidney, liver, lung, salivary gland and skeletal muscle tissue, with the highest expression in kidney. Biallelically expressed in placenta and fetal tissues.
  • Pathway

    Amine and polyamine biosynthesis; creatine biosynthesis; creatine from L-arginine and glycine: step 1/2.
  • Involvement in disease

    Defects in GATM are the cause of arginine:glycine amidinotransferase deficiency (AGAT deficiency) [MIM:612718]. AGAT deficiency is an autosomal recessive disorder characterized by developmental delay/regression, mental retardation, severe disturbance of expressive and cognitive speech, and severe depletion of creatine/phosphocreatine in the brain.
  • Sequence similarities

    Belongs to the amidinotransferase family.
  • Domain

    One chain folds into a compact single domain composed of repeating units, five beta-beta-alpha-beta modules, which surround the central active site.
  • Cellular localization

    Mitochondrion inner membrane. Cytoplasm. The mitochondrial form is found in the intermembrane space probably attached to the outer side of the inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGAT antibody
    • AT antibody
    • GATM antibody
    • GATM_HUMAN antibody
    • Glycine amidinotransferase antibody
    • Glycine amidinotransferase, mitochondrial antibody
    • L-arginine:glycine amidinotransferase antibody
    • mitochondrial antibody
    • Transamidinase antibody
    see all

Images

  • All lanes : Anti-GATM antibody [OTI1C9] (ab119269) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs
    Lane 2 : HEK-293T lysate transfected with pCMV6-ENTRY GATM cDNA for 48 hrs

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 48 kDa

  • Paraffin-embedded human kidney tissue stained for GATM using ab119269 at 1/100 dilution in immunohistochemical analysis.

     

  • Paraffin-embedded human liver tissue stained for GATM using ab119269 at 1/100 dilution in immunohistochemical analysis.

     

  • Paraffin-embedded human liver carcinoma tissue stained for GATM using ab119269 at 1/100 dilution in immunohistochemical analysis.

     

  • Paraffin-embedded human pancreas tissue stained for GATM using ab119269 at 1/100 dilution in immunohistochemical analysis.

     

  • COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY GATM, stained for GATM (Green) using ab119269 at 1/100 dilution in ICC/IF.

     

  • Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with GATM (Red), or an empty vector control plasmid (Blue), stained with ab119269 at 1/100 dilution.

References

ab119269 has not yet been referenced specifically in any publications.

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