Application
Western blot
Sample
Rat Tissue lysate - whole (Brain)
Loading amount
20 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Concentration
1 µg/ml
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Dako Cytomation P0260
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL plus
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 60 kDa Non-specific: 78 kDa
Positive control
Brain
Additional data
Additional Notes
Western Blot 10% PAGE probing with Mouse monoclonal GBA (Ab55080) antibody. Lane 1: Adult rat cortex, lane 2:adult rat striatum, Lane 3: adult rat cerebellum, Lane 4: adult rat liver. Lane 5: mouse brain. A specific 60kDA band detected in all brain samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Ruma Raha-Chowdhury
Verified customer
Submitted Sep 07 2010