• Product name
  • Description
    Mouse monoclonal to GBA
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (GST-tag) corresponding to Human GBA aa 146-236.




Our Abpromise guarantee covers the use of ab55080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 60 kDa.
IHC-P Use a concentration of 3 µg/ml.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.


  • Involvement in disease
    Defects in GBA are the cause of Gaucher disease (GD) [MIM:230800]; also known as glucocerebrosidase deficiency. GD is the most prevalent lysosomal storage disease, characterized by accumulation of glucosylceramide in the reticulo-endothelial system. Different clinical forms are recognized depending on the presence (neuronopathic forms) or absence of central nervous system involvement, severity and age of onset.
    Defects in GBA are the cause of Gaucher disease type 1 (GD1) [MIM:230800]; also known as adult non-neuronopathic Gaucher disease. GD1 is characterized by hepatosplenomegaly with consequent anemia and thrombopenia, and bone involvement. The central nervous system is not involved.
    Defects in GBA are the cause of Gaucher disease type 2 (GD2) [MIM:230900]; also known as acute neuronopathic Gaucher disease. GD2 is the most severe form and is universally progressive and fatal. It manifests soon after birth, with death generally occurring before patients reach two years of age.
    Defects in GBA are the cause of Gaucher disease type 3 (GD3) [MIM:231000]; also known as subacute neuronopathic Gaucher disease. GD3 has central nervous manifestations.
    Defects in GBA are the cause of Gaucher disease type 3C (GD3C) [MIM:231005]; also known as pseudo-Gaucher disease or Gaucher-like disease.
    Defects in GBA are the cause of Gaucher disease perinatal lethal (GDPL) [MIM:608013]. It is a distinct form of Gaucher disease type 2, characterized by fetal onset. Hydrops fetalis, in utero fetal death and neonatal distress are prominent features. When hydrops is absent, neurologic involvement begins in the first week and leads to death within 3 months. Hepatosplenomegaly is a major sign, and is associated with ichthyosis, arthrogryposis, and facial dysmorphism.
    Note=Perinatal lethal Gaucher disease is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
    Defects in GBA contribute to susceptibility to Parkinson disease (PARK) [MIM:168600]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. Additional features are characteristic postural abnormalities, dysautonomia, dystonic cramps, and dementia. The pathology of Parkinson disease involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. The disease is progressive and usually manifests after the age of 50 years, although early-onset cases (before 50 years) are known. The majority of the cases are sporadic suggesting a multifactorial etiology based on environmental and genetic factors. However, some patients present with a positive family history for the disease. Familial forms of the disease usually begin at earlier ages and are associated with atypical clinical features.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 30 family.
  • Cellular localization
    Lysosome membrane. Interaction with saposin-C promotes membrane association.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acid beta glucosidase antibody
    • Acid beta-glucosidase antibody
    • Alglucerase antibody
    • Beta glucocerebrosidase antibody
    • Beta-glucocerebrosidase antibody
    • betaGC antibody
    • D glucosyl N acylsphingosine glucohydrolase antibody
    • D-glucosyl-N-acylsphingosine glucohydrolase antibody
    • EC antibody
    • GBA antibody
    • Gba protein antibody
    • GBA1 antibody
    • GC antibody
    • GCase antibody
    • GCB antibody
    • GLCM_HUMAN antibody
    • GLUC antibody
    • Glucocerebrosidase (alt.) antibody
    • Glucocerebrosidase antibody
    • Glucosidase beta antibody
    • Glucosidase, beta, acid antibody
    • Glucosidase, beta; acid (includes glucosylceramidase) antibody
    • Glucosylceramidase antibody
    • Imiglucerase antibody
    • Lysosomal glucocerebrosidase antibody
    • OTTHUMP00000033992 antibody
    • OTTHUMP00000033993 antibody
    see all


  • Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
    Lane 2: GBA knockout HAP1 whole cell lysate (40 µg)
    Lane 3: MCF7 whole cell lysate (40 µg)
    Lane 4: HepG2 whole cell lysate (40 µg) 

    Lanes 1 - 4: Merged signal (red and green). Green - ab55080 observed at 70 kDa. Red - loading control, ab181602, observed at 37 kDa. 

    ab55080 was shown to specifically react with GBA in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when GBA knockout samples were used. Wild-type and GBA knockout samples were subjected to SDS-PAGE. Ab55080 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • ab55080 at 10 ug/ml staining GBA in human Hela cells by Immunocytochemistry/ Immunofluorescence.

  • GBA antibody (ab55080) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
  • GBA antibody (ab55080) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human breast cancer.
  • All lanes : Anti-GBA antibody (ab55080) at 1/1000 dilution

    Lane 1 : Lysate prepared from MOCK
    Lane 2 : Lysate prepared from human HN10 cells

    Lysates/proteins at 10 µg per lane.

    All lanes : IRDye® donkey polyclonal to mouse IgG at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa

    Exposure time: 1 minute

    See Abreview


This product has been referenced in:
  • Dopeso-Reyes IG  et al. Glucocerebrosidase expression patterns in the non-human primate brain. Brain Struct Funct 223:343-355 (2018). IHC-FrFl . Read more (PubMed: 28835999) »
  • Yang C  et al. Celastrol increases glucocerebrosidase activity in Gaucher disease by modulating molecular chaperones. Proc Natl Acad Sci U S A 111:249-54 (2014). Read more (PubMed: 24351928) »
See all 7 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A


Thank you for contacting us and sorry for the delay in getting back to you.

We are not able to provide SDS documents in Italian but the English version can now be accessed from the datasheet of ab55080, or you will find it attached to this email.

I hope this has been of help If you have any further requests, please do not hesitate to contact us again.

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Thank you for your email.

This antibody does not have any hazardous material in it so MSDS will not make any sense. For antibodies where hazardous material is used to preserve the solution the MSDS can be downloaded from online datasheet.

I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

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Thank you for contacting us.

We unfortunately do not provide MSDS in Italian language. We have facility to provide MSDS in Chinese, Japanese, English, Spanish, French and German languages but not Italian.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!

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Thank you for your response.

A lysate ofPeptostreptococcus magnus,purified Protein L protein or recombinant Protein Lwould all be ideal positive controls for use withab63506.

Fora positive control to use with our anti GBA antibody, ab55080. We have data on our website which shows the antibody to work withMCF-7 cell lysate and withHN10 cells. I would recommend one of those lysates for a positive control.

I would like to encourage you to take advantage of our Abreview program. It takes just a few minutes to leave a review and you can collect Abpoints which you may redeem for Abcam products or Amazon gift cards while at the same time sharing information about the product with your colleagues worldwide. More information may be at:


I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Thank you for contacting us.

When choosing a loading control it is best practice to choose housekeeping genes which will be present in your sample type. For example, in whole cell preparations beta Actin or GAPDH will be appropriate while in nuclear or mitochondrial fractions use Lamin B1 or COXIV respectively.

These controls are excellent when you sample is eukaryotic. However, as Protein L will be from bacteria these housekeeping proteins may not be present. I have found however that GAPDH may be found in E.coli.

I would recommend therefore that if your sample types are whole cell/membrane or cytoplasmic and are either bacterial (in the case of the Protein L antibody ab63506) or eukaryotic (as for use with anti GBA antibody ab55080) that you use our anti-GAPDH antibodies.

We do have one anti-GAPDH product which is guaranteed to work with E.coli, Ab85760. Further information about this product may be found at the following page:


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us. I am sorry to hear that ab55080 is not providing satisfactory results in WB.  Having reviewed this case, I would like to offer some suggestions to help optimize your results.  I would also appreciate if you can confirm some further details: 1.)  When you say that the detection was done with AP, did it involve ECL reagents or was the detection colorometric?  We find ECL to be much more sensitive than colorometric detection, and we use ECL for our in house WB validation.  2.)  It may help to use a lower percentage of milk in your antibody diluent.  I typically recommend 0.1 - 1 % milk in the antibody diluent because higher concentrations can prevent the antibody from binding effectively to the protein of interest.  3.)  What secondary antibody was used and at what dilution?  For weak signal, it can sometimes help to use more secondary antibody.  Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.  

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 949227. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research. PS: As discussed please try adding 50% Glycerol or 3% BSA before freezing these antibodies.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rat Tissue lysate - whole (Brain)
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 07 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Human Tissue sections (Brain section)
Brain section
Yes - 0.1x PBS plus 0.3xTriton x
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (HN10 cells)
Loading amount
10 µg
HN10 cells
transfected with pcDNA-GBA
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 10 2009


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