Recombinant Anti-GBA2 antibody [4A12] (ab252831)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [4A12] to GBA2
- Suitable for: IHC-P
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-GBA2 antibody [4A12]
See all GBA2 primary antibodies -
Description
Rat monoclonal [4A12] to GBA2 -
Host species
Rat -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: ICC/IF or IHC-Fr -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse testis and cerebrum tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4A12 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 0.023 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Heat mediated antigen retrieval using ab93684. |
Notes |
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IHC-P
Use a concentration of 0.023 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Heat mediated antigen retrieval using ab93684. |
Target
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Relevance
GBA2 is a non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide to free glucose and ceramide. It is involved in sphingomyelin generation and prevention of glycolipid accumulation. GBA2 may also catalyze the hydrolysis of bile acid 3-O-glucosides, however, the relevance of such activity is unclear in vivo. -
Cellular localization
Cell membrane; Single-pass membrane protein. Note=Not localized to lipid rafts. -
Database links
- Entrez Gene: 230101 Mouse
- SwissProt: Q69ZF3 Mouse
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Alternative names
- AD035 antibody
- Beta glucocerebrosidase 2 antibody
- Beta glucosidase 2 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling GBA2 with ab252831 at 0.023µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Membranous staining on mouse testis is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling GBA2 with ab252831 at 0.023µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse cerebrum is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab252831 has not yet been referenced specifically in any publications.