Anti-GBA2 antibody (ab205064)
Key features and details
- Rabbit polyclonal to GBA2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-GBA2 antibody
See all GBA2 primary antibodies -
Description
Rabbit polyclonal to GBA2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human GBA2 aa 700 to the C-terminus. (BC011363).
Database link: Q9HCG7 -
Positive control
- HeLa and HepG2 cell lysates; Human fetal colon tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Lyophilized:Reconstitute in 200ul sterile H2O. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500.
|
|
WB |
1/200 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 104 kDa).
|
Notes |
---|
IHC-P
1/100 - 1/500. |
WB
1/200 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 104 kDa). |
Target
-
Relevance
GBA2 is a non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide to free glucose and ceramide. It is involved in sphingomyelin generation and prevention of glycolipid accumulation. GBA2 may also catalyze the hydrolysis of bile acid 3-O-glucosides, however, the relevance of such activity is unclear in vivo. -
Cellular localization
Cell membrane; Single-pass membrane protein. Note=Not localized to lipid rafts. -
Database links
- Entrez Gene: 57704 Human
- Entrez Gene: 230101 Mouse
- GenBank: NM_020944.2 Human
- Omim: 609471 Human
- SwissProt: Q9HCG7 Human
- SwissProt: Q69ZF3 Mouse
-
Alternative names
- AD035 antibody
- Beta glucocerebrosidase 2 antibody
- Beta glucosidase 2 antibody
see all
Images
-
All lanes : Anti-GBA2 antibody (ab205064) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Predicted band size: 104 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal colon tissue labeling GBA2 with ab205064 at 1/100 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab205064 has been referenced in 1 publication.
- Akiyama H et al. Glucocerebrosidases catalyze a transgalactosylation reaction that yields a newly-identified brain sterol metabolite, galactosylated cholesterol. J Biol Chem 295:5257-5277 (2020). PubMed: 32144204