Anti-GBAS antibody (ab153833)
Key features and details
- Rabbit polyclonal to GBAS
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GBAS antibody
See all GBAS primary antibodies -
Description
Rabbit polyclonal to GBAS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment corresponding to a region within amino acids 1-225 of Human GBAS (Uniprot ID O75323)
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Positive control
- A549, H1299 and HCT116 whole cell lysate; Colon cancer tissue; Human muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Tissue specificity
Widely expressed. Most abundant in heart and skeletal muscle. -
Sequence similarities
Belongs to the NipSnap family. - Information by UniProt
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Database links
- Entrez Gene: 767938 Cow
- Entrez Gene: 2631 Human
- Entrez Gene: 14467 Mouse
- Entrez Gene: 498174 Rat
- Omim: 603004 Human
- SwissProt: O75323 Human
- SwissProt: O55126 Mouse
- Unigene: 591069 Human
see all -
Alternative names
- 4 nitrophenylphosphatase domain and non neuronal SNAP25 like 2 antibody
- gbas antibody
- Glioblastoma amplified sequence antibody
see all
Images
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All lanes : Anti-GBAS antibody (ab153833) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : H1299 cell lysate
Lane 3 : HCT116 cell lysate
Predicted band size: 34 kDa
12% SDS PAGE
30 µg of whole cell lysate -
Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue, using ab153833 antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded mouse Muscle, using ab153833 antibody at 1:500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab153833 has been referenced in 2 publications.
- Peng J et al. Knockdown of GBAS regulates esophageal cancer cell viability and apoptosis. Mol Med Rep 24:N/A (2021). PubMed: 34036378
- Princely Abudu Y et al. NIPSNAP1 and NIPSNAP2 Act as "Eat Me" Signals for Mitophagy. Dev Cell 49:509-525.e12 (2019). PubMed: 30982665