Recombinant
RabMAb

Recombinant Anti-Gbeta5 antibody [EPR14778] (ab185206)

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Overview

  • Product name

    Anti-Gbeta5 antibody [EPR14778]
    See all Gbeta5 primary antibodies

  • Description

    Rabbit monoclonal [EPR14778] to Gbeta5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Gbeta5 aa 50-150. The exact sequence is proprietary.
    Database link: O14775

  • Positive control

    • Human cerebellum, rat and mouse brain lysates; Human lung adenocarcinoma and mouse brain tissues; HACAT and U87-MG cells.

  • General notes

     

     

    Previously labelled as GNB5.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14778

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 44 kDa).
ICC/IF 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/40 - 1/60.
Flow Cyt 1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

  • Tissue specificity

    Expressed in multiple tissues.

  • Sequence similarities

    Belongs to the WD repeat G protein beta family.
    Contains 7 WD repeats.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • FLJ37457 antibody
    • FLJ43714 antibody
    • G protein beta 5 subunit antibody
    • G protein beta subunit 5L antibody
    • GB 5 antibody
    • GB5 antibody
    • GBB5_HUMAN antibody
    • Gbeta5 antibody
    • GNB 5 antibody
    • GNB5 antibody
    • Guanine nucleotide binding protein (G protein) beta 5 antibody
    • Guanine nucleotide binding protein beta 5 antibody
    • Guanine nucleotide binding protein beta 5 subunit antibody
    • Guanine nucleotide binding protein beta subunit 5L antibody
    • Guanine nucleotide binding protein subunit beta 5 antibody
    • Guanine nucleotide-binding protein subunit beta-5 antibody
    • Transducin beta chain 5 antibody
    see all

Images

  • Western blot - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Western blot - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Anti-Gbeta5 antibody [EPR14778] (ab185206) at 1/2000 dilution + Human cerebellum lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbeta5 antibody [EPR14778] (ab185206)

    Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling Gbeta5 with ab185206 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Immunoprecipitation - Anti-Gbeta5 antibody [EPR14778] (ab185206)

    Western blot analysis of Gbeta5 in immunoprecipitation pellets from Rat brain lysate using ab185206 at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

  • Flow Cytometry - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Flow Cytometry - Anti-Gbeta5 antibody [EPR14778] (ab185206)

    Flow cytometry analysis of 2% paraformaldehyde fixed HACAT cells labeling Gbeta5 with ab185206 at 1/160 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Immunocytochemistry/ Immunofluorescence - Anti-Gbeta5 antibody [EPR14778] (ab185206)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed U87-MG cells labeling Gbeta5 with ab185206 at 1/100 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counterstained with Dapi blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbeta5 antibody [EPR14778] (ab185206)

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Gbeta5 with ab185206 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Western blot - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Anti-Gbeta5 antibody [EPR14778] (ab185206) at 1/10000 dilution + Mouse brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa

  • Western blot - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Western blot - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Anti-Gbeta5 antibody [EPR14778] (ab185206) at 1/10000 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa

References

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