• Product name

  • Description

    Rabbit polyclonal to GBF1
  • Host species

  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 1809-1859 of Human GBF1 (NP_004184.1, GeneID 8729).

  • Positive control

    • Human Controls: Colon Carcinoma, Prostate Carcinoma Mouse Controls: Squamous Cell Carcinoma



Our Abpromise guarantee covers the use of ab105111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.


  • Function

    Guanine-nucleotide exchange factor (GEF) for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway; its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites (ERES), and the endoplasmic reticulum-Golgi intermediate (ERGIC) and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmatic reticulum (ER) (PubMed:16926190, PubMed:17956946, PubMed:18003980, PubMed:12047556, PubMed:12808027, PubMed:19039328, PubMed:24213530). Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adaptor protein complex related to chlathrin-dependent transport; the function requires its GEF activity (probably at least in part on ARF4 and ARF5) (PubMed:23386609). Has GEF activity towards ARF1 (PubMed:15616190). Has in vitro GEF activity towards ARF5 (By similarity). Involved in the processing of PSAP (PubMed:17666033). Required for the assembly of the Golgi apparatus (PubMed:12808027, PubMed:18003980). The AMPK-phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions (PubMed:18063581, PubMed:23418352). May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets; however, this function is under debate (PubMed:19461073, PubMed:22185782). In neutrophils, involved in G protein-coupled receptor (GPCR)-mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADPH oxidase complex (PubMed:22573891).
  • Tissue specificity

  • Sequence similarities

    Contains 1 SEC7 domain.
  • Domain

    The DCB (dimerization and cyclophiln-binding) and HUS (homology upstream of Sec7) domains are necessary for dimerization. The DCB domain is proposed to support constitutive homodimerization; the HUS domain interacts with the DCB domain which may occur intramolecular or intermolecuar.
  • Post-translational

    AMPK-mediated phosphorylation at Thr-1337 is induced by 2-deoxyglucose (2-DG) and AICA ribonucleotide, and occurs during mitosis leading to membrane disassociation and inactivation of ARF1 during mitosis.
  • Cellular localization

    Golgi apparatus, cis-Golgi network. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus, trans-Golgi network. Cytoplasm. Lipid droplet. Membrane. Cycles rapidly on and off early Golgi membranes (PubMed:15616190). Stabilized on membranes when complexed with ARF1-GDP and is released from both ARF1 and membranes after it catalyzes GDP displacement and ARF1 binds GTP. Continuous cycles of recruitment and dissociation of GBF1 to membranes are required for sustained ARF activation and COP I recruitment (PubMed:15813748). In neutrophils is translocated from the Golgi to the leading edge upon GPCR stimulation (PubMed:22573891). Localization to lipid droplets is questionable (PubMed:22185782).
  • Information by UniProt
  • Database links

  • Alternative names

    • ARF1GEF antibody
    • BFA resistant GEF 1 antibody
    • BFA-resistant GEF 1 antibody
    • FLJ21263 antibody
    • FLJ21500 antibody
    • GBF1 antibody
    • GBF1_HUMAN antibody
    • golgi brefeldin A resistant guanine nucleotide exchange factor 1 antibody
    • Golgi specific brefeldin A resistance factor 1 antibody
    • Golgi specific Brefeldin A-resistance factor antibody
    • Golgi-specific brefeldin A-resistance guanine nucleotide exchange antibody
    • Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 antibody
    • KIAA0248 antibody
    • MGC134877 antibody
    • MGC134878 antibody
    see all


  • ab105111, at a 1/250 dilution, staining GBF1 in formalin fixed, paraffin embedded Human prostate carcinoma tissue by Immunohistochemistry. Detection: DAB staining
  • ab105111, at a 1/250 dilution, staining GBF1 in formalin fixed, paraffin embedded Mouse squamous cell carcinoma tissue by Immunohistochemistry. Detection: DAB staining


This product has been referenced in:

  • Wang J  et al. The Arf GEF GBF1 and Arf4 synergize with the sensory receptor cargo, rhodopsin, to regulate ciliary membrane trafficking. J Cell Sci 130:3975-3987 (2017). Read more (PubMed: 29025970) »
See 1 Publication for this product

Customer reviews and Q&As


Thank you for contacting us. We have two antibodies in our catalogue against GBF1. Both are rabbit polyclonals, ab86071 and ab105111. ab86071 has been tested with human and mouse samples with immunoprecipitation and Western blotting. ab105111 again is reactive against both human and mouse GBF1 and has been previously used in IHC-P. We have several antibodies directed against HuR, with two mouse monoclonals with similar profiles to Sc-5261. Both Anti-HuR / ELAVL1 antibody [19F12] (ab14371) and [4G8] (ab110081) have been used in immunocytochemistry, IHC (frozen and paraffin sections) and Western blotting and are reactive with human samples (ab14371 also recognises the mouse antigen). All our antibodies are covered by the Abpromise guarantee. This means that we guarantee that our products work in the tested species and applications stated on the datasheet and we replace or fully refund products not performing as stated on the datasheet when reported within 6 months of delivery. More information on the Abpromise can be found here: www.abcam.com/abpromise I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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