Overview

  • Product name

    Anti-GBP1 antibody [EPR8285]
    See all GBP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8285] to GBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GBP1 aa 550-650. The exact sequence is proprietary.

  • Positive control

    • HeLa cell lysates (with and without treatment with IFN gamma; Human spleen tissue; IFN gamma induced HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Binds GTP, GDP and GMP.
    • Sequence similarities

      Belongs to the GBP family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • GBP 1 antibody
      • GBP-1 antibody
      • GBP1 antibody
      • GBP1_HUMAN antibody
      • GTP binding protein 1 antibody
      • GTP-binding protein 1 antibody
      • Guanine nucleotide binding protein 1 antibody
      • Guanine nucleotide-binding protein 1 antibody
      • Guanylate binding protein 1 antibody
      • Guanylate binding protein 1 interferon inducible 67kDa antibody
      • Guanylate binding protein 1 interferon inducible antibody
      • HuGBP 1 antibody
      • HuGBP-1 antibody
      • HuGBP1 antibody
      • Interferon induced guanylate binding protein 1 antibody
      • Interferon-induced guanylate-binding protein 1 antibody
      • OTTHUMP00000012352 antibody
      see all

    Images

    • All lanes : Anti-GBP1 antibody [EPR8285] (ab131255) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, treated with IFN gamma
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 68 kDa

    • Immunofluorescent analysis of IFN-gamma induced HeLa cells labelling GBP1 with ab131255 antibody at 1/100 dilution.
    • Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling GBP1 with ab131255 antibody at 1/50 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Halu A  et al. Context-enriched interactome powered by proteomics helps the identification of novel regulators of macrophage activation. Elife 7:N/A (2018). Read more (PubMed: 30303482) »
    • Piro AS  et al. Detection of CytosolicShigella flexnerivia a C-Terminal Triple-Arginine Motif of GBP1 Inhibits Actin-Based Motility. MBio 8:N/A (2017). Read more (PubMed: 29233899) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131255.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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